2014-2015年广州地区腹泻患儿和健康儿童致泻性大肠埃希菌流行特征及耐药分析

发布时间：2018-04-18 12:43

本文选题：腹泻 + 肠致病性大肠杆菌　；参考：《中华疾病控制杂志》2016年04期

【摘要】：目的了解广州地区致泻性大肠埃希菌感染情况、流行病学特征及耐药情况。方法 2014年7月~2015年7月分别收集广州地区某综合医院腹泻患儿及健康儿童粪便标本143份、83份,进行细菌分离培养,采用聚合酶链反应检测肠致病性大肠埃希菌、聚集性大肠埃希菌、产毒性大肠埃希菌和肠侵袭性大肠埃希菌,Kirby Bauer法(K-B纸片扩散法)进行药敏试验。结果四种致泻性大肠埃希菌在腹泻患儿与健康儿童中的检出率分别为1.4%、3.6%;2.8%、2.4%;1.4%、1.2%;0.7%、1.2%;腹泻患儿3~7岁年龄段检出率最高,占26.7%;然而,健康儿童中5~7岁年龄段检出率最高,占8.4%。药敏试验结果显示,大肠埃希菌对四环素、氨苄西林耐药率为52.6%~63.2%;头孢类耐药率为5.3%~21.1%;多重耐药率50.5%。结论致泻性大肠埃希菌在腹泻患儿及健康儿童均有检出,并存在多重感染,提示致泻性大肠埃希菌是机会感染菌;除此之外,耐药及多重耐药高,应加强致泻性大肠埃希菌监测及管理抗生素使用。
[Abstract]:Objective to investigate the prevalence, epidemiological characteristics and drug resistance of diarrhea Escherichia coli in Guangzhou.Methods from July 2014 to July 2015, 143 fecal specimens of diarrhea children and healthy children were collected from a general hospital in Guangzhou area. The bacteria were isolated and cultured. Escherichia coli was detected by polymerase chain reaction (PCR).The drug sensitivity test was carried out by Kirby Bauer method and K-B disk diffusion method for agglomeration Escherichia coli, toxic Escherichia coli and enteric invasive Escherichia coli.Results the detection rates of four kinds of diarrhea Escherichia coli in diarrhea children and healthy children were 1.4 and 1.4, respectively. The detection rate of four kinds of diarrhea was 1.4 and 1.20.The detection rate of diarrhea was the highest in the age group of 3 to 7 years old, and the highest rate was in the age of 57 years old, accounting for 8.4% of the total number of healthy children.The results of drug sensitivity test showed that the resistance rate of Escherichia coli to tetracycline and ampicillin was 52.63.2cm, the resistance rate of cephalosporins was 5.3cm and the multidrug resistance rate was 50.5.Conclusion diarrhea Escherichia coli was detected in both children with diarrhea and healthy children, and had multiple infections, suggesting that diarrhea Escherichia coli is opportunistic infection, in addition, drug resistance and multidrug resistance are high.The surveillance and management of antibiotic use should be strengthened.
【作者单位】：南方医科大学公共卫生与热带医学学院流行病学教研室;
【分类号】：R725.1;R446.5

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