宠物源大肠埃希菌的分离鉴定和耐药性研究

发布时间：2018-04-18 22:25

本文选题：大肠埃希菌 + 耐药性　；参考：《动物医学进展》2016年02期

【摘要】：为调查长春地区宠物源大肠埃希菌耐药流行情况,从2家宠物医院采集135份宠物肛拭子样品,分离和鉴定大肠埃希菌并进行多重PCR分群。测定大肠埃希菌分离株对19种抗菌药物的耐药性,并鉴定超广谱β-内酰胺酶(ESBLs)表型。共分离鉴定获得95株宠物源大肠埃希菌,它们对氨苄西林和哌拉西林的耐药率最高(78.9%和76.8%);其次是四环素(61.6%);对头孢唑林、头孢噻肟、头孢吡肟、庆大霉素、复方新诺明、环丙沙星和左氧氟沙星的耐药率均超过了50%;所有分离株均对亚胺培南和美罗培南敏感。其中15个分离株对受试的抗菌药物全部敏感(15.8%),63株呈多重耐药表型(66.3%)。ESBLs型菌株占53.7%。本研究探明了长春地区宠物源大肠埃希菌的耐药流行情况,对宠物临床上用药具有指导价值。
[Abstract]:In order to investigate the drug resistance of Escherichia coli from pet origin in Changchun area, 135 anal swab samples were collected from 2 pet hospitals. Escherichia coli was isolated, identified and divided into multiple PCR groups.The resistance of Escherichia coli isolates to 19 antimicrobial agents and the phenotype of extended-spectrum 尾 -lactamases (ESBLs) were determined.A total of 95 strains of Escherichia coli from pet were isolated and identified. Their resistance rates to ampicillin and piperacillin were the highest, 78.9% and 76.810%, followed by tetracycline 61.6%, cefazolin, cefotaxime, cefepime, gentamicin, compound nephrine,The drug resistance rates of ciprofloxacin and levofloxacin were higher than 50 and all isolates were sensitive to imipenem and meropenem.Among them, 15 isolates were all sensitive to antimicrobial agents. 63 strains showed multidrug resistance phenotype (66.3%). ESBLs-type strains accounted for 53.7%.This study has proved the drug resistance of Escherichia coli from pets in Changchun area, which has guiding value for clinical use of drugs in pets.
【作者单位】：军事医学科学院军事兽医研究所吉林省人兽共患病预防与控制重点实验室;
【基金】：国家科技部传染病重大专项(2013ZX10004-217-002) 863项目(2012AA022006) 吉林省科技计划项目(20150101110JC)
【分类号】：R446.5

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