鲍曼不动杆菌细胞内的舒巴坦浓度与外膜蛋白表达相关性研究

发布时间：2018-04-18 22:40

本文选题：鲍曼不动杆菌 + 舒巴坦　；参考：《中国临床药理学杂志》2016年20期

【摘要】：目的探索鲍曼不动杆菌外膜孔道蛋白介导的耐药机制,研究碳青霉烯耐药相关性外膜蛋白(Car O)在舒巴坦耐药中的地位与作用。方法测定临床分离的鲍曼不动杆菌的最低抑菌浓度(MIC),用液质联用法测定菌体内舒巴坦浓度,并对外膜蛋白提取后进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),测定Car O的表达情况,分析外膜蛋白与菌体内舒巴坦浓度间关系。结果舒巴坦对5株临床分离的鲍曼不动杆菌58C5、878-13、8F3、161-20、373-58的MIC分别为16,4,32,8,16μg·m L~(-1),相应外膜蛋白29 k Da条带的光密度值分别为1.00,2.02,0.33,1.80,0.85。29 k Da条带光密度值与胞内舒巴坦浓度存在显著相关性(P0.01)。结论 Car O低表达可能是舒巴坦对鲍曼不动杆菌MIC值升高的机制之一。
[Abstract]:Objective to explore the mechanism of drug resistance mediated by extracellular pore protein of Acinetobacter baumannii and to study the role of carbapenem resistance-associated outer membrane protein (car O) in sulbactam resistance.Methods the minimal inhibitory concentration of Acinetobacter baumannii was determined and the concentration of sulbactam in the bacteria was determined by HPLC.The external membrane protein was extracted by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGEN) to determine the expression of Car O and to analyze the relationship between the outer membrane protein and the concentration of sulbactam in the bacteria.Results the MIC of sulbactam against 5 clinical isolates of Acinetobacter baumannii 58C5878-138F3161-20373-58 was 164P0.01a, and the optical density of the corresponding 29kDa band of outer membrane protein was 1.002.020.33.31.80 kDa and the intracellular concentration of sulbactam was significantly correlated with the intracellular sulbactam concentration (P0.01a). The results showed that there was a significant correlation between the optical density and the intracellular sulbactam concentration of Acinetobacter baumannii (58C5878-138F3161-20373-58).Conclusion the low expression of Car O may be one of the mechanisms by which sulbactam increases the MIC value of Acinetobacter baumannii.
【作者单位】：南京大学医学院附属口腔医院药学部;南京医科大学第一附属医院检验学部;
【基金】：南京市科技发展基金资助项目(201503066) 南京市医学科技发展基金资助项目(QYK11130)
【分类号】：R446.5

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