临床分离金黄色葡萄球菌生物膜形成、毒性以及耐药性的研究

发布时间：2018-04-22 09:07

本文选题：金黄色葡萄球菌 + 耐药性　；参考：《现代预防医学》2016年07期

【摘要】：目的分析临床分离金黄色葡萄球菌(Staphylococcus aureus,S.aureus)的耐药性,毒性和生物膜(Bacterial biofilm,BF)形成能力,并进一步探究三者之间的关系,为临床预防和治疗S.aureus感染提供理论依据。方法临床分离的122株S.aureus经全自动微生物分析仪鉴定及药物敏感性检测,采用结晶紫染色和刚果红平板法分析生物膜形成能力;通过对其溶血素、胞外蛋白酶和脂肪酶的检测评估其毒性。结果 122株S.aureus甲氧西林耐药株(MRSA)分离率达到将近70%,且表现出多重耐药现象;31.97%能产生溶血素,29.51%能产生胞外蛋白酶,54.10%能产生胞外脂肪酶;约40%的菌株有一定的生物膜形成能力,且MRSA的生物膜形成能力强于甲氧西林敏感株(MSSA),毒性弱于MSSA。结论 S.aureus感染引起的不同临床疾病主要与其产生的毒素和耐药性有关,而细菌的生物膜对于其毒性和耐药性同样有很重要的影响。
[Abstract]:Objective to analyze the drug resistance, toxicity and biofilm formation ability of Staphylococcus aureus Staphylococcus aureus in clinical isolation, and to explore the relationship between them and to provide theoretical basis for clinical prevention and treatment of S.aureus infection. Methods 122 clinical isolates of S.aureus were identified by automatic microbiological analyzer and drug sensitivity was detected. The biofilm forming ability was analyzed by crystal violet staining and Congo red plate method. The toxicity of extracellular protease and lipase was evaluated. Results the isolation rate of 122 S.aureus methicillin-resistant strains was nearly 70, and showed that 31.97% of the strains could produce hemolysin and 54.10% extracellular lipase, and about 40% of the strains had the ability to form biofilm. The biofilm forming ability of MRSA was stronger than that of methicillin-sensitive strain MSSAA, and its toxicity was weaker than that of MSSA-sensitive strain. Conclusion the different clinical diseases caused by S.aureus infection are mainly related to the toxin production and drug resistance, and the biofilm of bacteria has an important effect on the toxicity and drug resistance.
【作者单位】：牡丹江师范学院;安徽省中医院检验科;中国科学技术大学;
【基金】：黑龙江省牡丹江师范学院省级重点预研项目(sy201230)
【分类号】：R446.5

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