B_w31的血型血清学和分子生物学研究——附1例报告

发布时间：2018-04-23 19:20

  本文选题：ABO亚型 + Bw　； 参考：《中国输血杂志》2016年10期

【摘要】：目的对1例正反定型不符的标本进行血清学疑难血型鉴定和分子生物学机制分析。方法应用常规血型血清学方法进行血型鉴定;对ABO基因的Exon6和Exon7扩增后进行直接测序,并进行单体型测序确定其基因型。结果该例标本血清学表现为B_w,红细胞与抗-A不凝集,与抗-B 2+凝集,与抗-A1不凝集,血清中存在抗-A;直接测序及单体型测序显示,此例标本的B等位基因在Exon7存在nt664GA突变。结论经测序检测证实,此例血清学表现为B_w的标本,其基因型为罕见的B_w31/O01型。
[Abstract]:Objective to identify the difficult blood group and analyze the molecular biological mechanism of a sample with positive and negative typing. Methods routine blood group serological method was used to identify the blood group, the Exon6 and Exon7 of ABO gene were amplified and sequenced directly, and their genotypes were determined by haplotype sequencing. Results the serological manifestations of this sample were: B _ W, erythrocyte and anti--A agglutination, anti-B _ 2 agglutination, anti-A _ 1 agglutination, and serum anti--A.The direct sequencing and haplotype sequencing showed that the B allele of this sample had nt664GA mutation in Exon7. Conclusion the serological features of this serological specimen were confirmed by sequencing, and the genotype of BW was a rare B_w31/O01 type.
【作者单位】： 日照市中心血站;青岛市中心血站输血研究所;
【分类号】：R457.11
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本文编号：1793268