不同血细胞分离机采集程序对健康供者造血干细胞采集物细胞成分的影响

发布时间：2018-04-24 20:40

  本文选题：外周血造血干细胞采集 + MNC程序　； 参考：《中国实验血液学杂志》2016年06期

【摘要】：目的:观察COBE Spectra血细胞分离机AutoPBSC程序以及Spectra Optia血细胞分离机MNC程序对采集的健康供者外周血造血干细胞成分的影响。方法:2015年1月至2016年8月期间对12例供者随机应用COBE Spectra血细胞分离机AutoPBSC程序以及Spectra Optia血细胞分离机MNC程序进行外周血造血干细胞采集,观察应用不同采集程序获得的采集物中单个核细胞、CD34~+细胞、粒细胞、淋巴细胞、血小板数的差异。结果:两种采集程序在循环血量、采集时间及抗凝剂使用量方面无显著差异。COBE Spectra血细胞分离机AutoPBSC程序采集的采集物体积及单个核细胞计数均低于Spectra Optia血细胞分离机MNC程序的采集物体积及单个核细胞计数。AutoPBSC程序采集的CD34~+细胞计数高于MNC程序采集的CD34~+细胞计数。AutoPBSC程序采集物中混入的淋巴细胞及血小板较MNC程序中多,其差异均有统计学意义(P0.05)。结论:与Spectra Optia血细胞分离机MNC程序相比,COBE Spectra血细胞分离机AutoPBSC程序采集的造血干细胞数量多,混入的淋巴细胞及血小板多。
[Abstract]:Aim: to observe the effect of AutoPBSC procedure of COBE Spectra blood cell separator and MNC procedure of Spectra Optia blood cell separator on the components of peripheral blood hematopoietic stem cells collected from healthy donors. Methods: from January 2015 to August 2016, 12 donors were randomly collected from peripheral blood hematopoietic stem cells by AutoPBSC program of COBE Spectra blood cell separator and MNC program of Spectra Optia blood cell separator. The number of CD34 ~ cells, granulocytes, lymphocytes and platelets in mononuclear cells were observed. Results: two kinds of collecting procedures were in circulation blood volume, There was no significant difference in collection time and anticoagulant usage. The volume of samples and the count of mononuclear cells collected by AutoPBSC program of COBE Spectra blood cell separator were lower than those of MNC program of Spectra Optia blood cell separator, and the count of mononuclear cells was lower than that of Spectra Optia blood cell separator. The number of CD34 ~ cells collected by AutoPBSC program was higher than that by MNC program. The number of lymphocytes and platelets mixed in the collection of AutoPBSC program was more than that in MNC program. The difference was statistically significant (P 0.05). Conclusion: compared with the MNC procedure of Spectra Optia blood cell separator, the number of hematopoietic stem cells collected by AutoPBSC program is more than that of MNC procedure, and the number of lymphocytes and platelets mixed with AutoPBSC program is more than that of MNC procedure.
【作者单位】： 西安交通大学第一附属医院血液内科;
【基金】：2016年陕西省自然科学基础研究计划青年基金项目(2016JQ8057)
【分类号】：R457.7
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本文编号：1798184