miR-34a与miR-125b在类风湿性关节炎患者外周血单个核细胞中的表达及临床意义

发布时间：2018-04-27 23:51

本文选题：关节炎 + 类风湿　；参考：《世界科技研究与发展》2015年03期

【摘要】：目的探讨miR-34a与miR-125b在类风湿性关节炎(RA)、骨性关节炎(OA)患者外周血单个核细胞(PBMCs)中的表达,并探讨其临床意义。方法以RA患者35例、OA患者15例及健康组20例为研究对象。取外周血,用淋巴细胞分离液分离出PBMCs,提取PBMCs中的总RNA,应用实时荧光定量聚合酶链反应检测miR-34a、miR-125b的表达水平,结合患者临床资料进行统计学分析。结果 RA患者miR-34a、miR-125b的表达水平低于健康组和OA组(P0.05),OA组miR-125b的表达水平低于健康组(P0.05)。RA患者miR-34a的表达水平与CRP呈正相关、与RF负相关、与ESR、anti-CCP无相关性,miR-125b与ESR、CRP呈正相关、与RF负相关、与anti-CCP无相关性。OA患者miR-34a、miR-125b的表达水平与ESR、CRP呈正相关,与RF、anti-CCP无相关性,这提示miR-34a及miR-125b的表达下调与RA和OA病情活动性可能相关。结论 RA患者外周血miR-34a和miR-125b表达下调,并与RA实验室指标有相关性,其表达水平可能与RA发生、发展、转化相关,为探讨miR-34a和miR-125b在类风湿关节炎中的作用及可能机制提供帮助。
[Abstract]:Objective to investigate the expression of miR-34a and miR-125b in peripheral blood mononuclear cells (PBMC) from patients with rheumatoid arthritis (RA) and its clinical significance. Methods 35 patients with RA and 15 patients with OA and 20 healthy subjects were studied. Peripheral blood was isolated from peripheral blood and PBMC cells were isolated from peripheral blood. The total RNAs in PBMCs were extracted. The expression level of miR-34asimiR-125b was detected by real-time fluorescence quantitative polymerase chain reaction. The clinical data of the patients were analyzed statistically. Results the expression level of miR-34a miR-125b in RA patients was lower than that in healthy controls and OA patients. The expression of miR-125b was positively correlated with CRP, negatively correlated with RF, and negatively correlated with CRP, and negatively correlated with RF, but positively correlated with ESRR-125b and negatively correlated with RF. The expression of miR-34a miR-125b was positively correlated with anti-CCP, but not correlated with anti-CCP, suggesting that the down-regulation of miR-34a and miR-125b might be related to the activity of RA and OA. Conclusion the expression of miR-34a and miR-125b in peripheral blood of RA patients is down-regulated and correlated with the laboratory parameters of RA. The expression level of miR-34a and miR-125b may be related to the occurrence, development and transformation of RA. It may be helpful to explore the role and possible mechanism of miR-34a and miR-125b in rheumatoid arthritis.
【作者单位】：兰州大学第二医院检验科;兰州大学第二医院骨科;
【基金】：甘肃省科技支撑项目(041255)资助
【分类号】：R593.22

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