重庆市大学生精液常规参数与精子DNA损伤的关联性分析

发布时间：2018-04-28 08:10

本文选题：精液分析 + 精子DNA损伤　；参考：《第三军医大学学报》2016年11期

【摘要】：目的探讨重庆市在校大学生精液常规参数与精子DNA损伤水平之间的关联性。方法 2014年6月招募的重庆大学生男性生殖健康调查(male reproductive health in Chongqing college students,MARHCS)队列639名健康大学生,手淫法采集精液样本,用计算机辅助精子分析系统(Computer-aided sperm analysis,CASA)进行精液常规分析,采用Diff-Quick试剂盒检测精子形态,采用单细胞凝胶电泳(彗星实验)检测精子DNA损伤,采用Spearman相关分析探讨精子DNA损伤与精液常规参数间的关联性。结果大学生人群精液常规各指标(中位数):精液量为3.6 m L,精子密度为52.0×106/m L,精子总活力为89.4%,前向运动精子百分率为57.0%,精子正常形态率为21.3%。精子DNA损伤(彗星实验指标)水平:尾部DNA含量百分比(Tail DNA%)为19.4%,尾长(tail length,TL)为35.7 pixels,彗星长(comet length,CL)为81.6 pixels,尾距(tail moment,TM)为7.6,Olive尾距(olive tail moment,OTM)为6.3。经Spearman相关性分析,精子密度与Tail DNA%呈负向关联(P0.05),精子总活力(%)与Tail DNA%、CL、TM、OTM呈负向关联(P值均0.01),前向运动精子百分率与精子Tail DNA%、CL、TL、TM、OTM呈负向关联(P值均0.05)。结论重庆市大学生精子密度、精子总活力、前向运动精子百分率均与精子DNA损伤呈负向关联。
[Abstract]:Objective to investigate the relationship between sperm routine parameters and sperm DNA damage level in college students in Chongqing. Methods A total of 639 male reproductive health in Chongqing college students were enrolled in the male reproductive health survey in Chongqing in June 2014. Semen samples were collected by masturbation, and semen routine analysis was performed by computer-aided sperm analysis system (Computer-aided sperm Analysis CASAA). Diff-Quick kit was used to detect sperm morphology, single cell gel electrophoresis (comet assay) was used to detect sperm DNA damage, and Spearman correlation analysis was used to study the relationship between sperm DNA damage and semen parameters. Results the parameters of semen routine (median: sperm volume 3.6 mL, sperm density 52.0 脳 10 6 / mL, total sperm motility 89.4), forward motility sperm percentage 57.0%, normal sperm morphology rate 21.3% were found in the college students (median sperm volume 3.6 mL), sperm density (52.0 脳 10 6 / mL), total sperm motility (89 .4), forward motility sperm percentage (57.0%) and normal sperm morphology. Sperm DNA damage (comet assay index): the percentage of tail DNA content and tail length were 19.4, 35.7 pixels, 81.6 pixels and 7.6 OTM respectively. According to Spearman correlation analysis, there was a negative correlation between sperm density and Tail% (P0.05, total sperm motility) and Tail Tail Tail TMOTM (P = 0.01). The percentage of forward motile spermatozoa was negatively correlated with Tail TLT TLT ~ (TM) OTM (P = 0.05). Conclusion the sperm density, total sperm motility and the percentage of forward motility spermatozoa were negatively correlated with sperm DNA damage in Chongqing university students.
【作者单位】：宁夏医科大学公共卫生与管理学院;第三军医大学军事预防医学院毒理学研究所;
【基金】：国家自然科学基金重点项目(81130051)~~
【分类号】：R446.19

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