鲍曼不动杆菌生物膜形成能力与生物膜相关基因及耐药性之间的关系

发布时间：2018-04-28 10:07

本文选题：鲍曼不动杆菌 + 生物膜　；参考：《青岛大学》2015年硕士论文

【摘要】：目的探讨鲍曼不动杆菌临床分离菌株生物膜形成能力及生物膜相关基因的分布情况,分析其与细菌耐药表型的关系。方法采用前瞻性研究,收集成武县人民医院2012年10月至2013年10月分离的70株鲍曼不动杆菌,采用96孔聚苯乙烯板构建生物膜模型,结晶紫染色法定性、定量分析生物膜形成能力,比较不同生物膜形成能力的鲍曼不动杆菌抗菌药物的耐药性。运用聚合酶链反应(PCR)技术扩增及检测生物膜相关蛋白Bap,生物膜合成相关基因bfs和Ⅰ类整合酶基因int I1的表达情况。结果70株鲍曼不动杆菌中40株为多重耐药菌,6株为泛耐药菌。68株(97.14%)具有生物膜形成能力,其中弱阳性14株,阳性20株,强阳性34株。鲍曼不动杆菌对亚胺培南、阿米卡星、美罗培南、头孢吡肟的耐药率分别为30.00%、32.86%、38.57%及41.43%,对其他常用抗菌药物的耐药率均不小于50%。且随着生物膜形成能力的增强,鲍曼不动杆菌对左氧氟沙星(χ2=9.225,P=0.010)、头孢吡肟(χ2=7.222,P=0.027)、庆大霉素(χ2=6.601,P=0.037)的耐药率显著降低。生物膜弱阳性、阳性和强阳性鲍曼不动杆菌Bap基因阳性率分别为50.00%、65.00%和79.41%(χ2=4.244,P=0.120),bfs阳性率分别为35.71%、65.00%和88.24%(χ2=13.602,P=0.001),int I1阳性率分别为42.86%、75.00%和91.18%(χ2=12.902,P=0.002)。生物膜相关基因阳性组对抗菌药物耐药率大多高于基因阴性组,其中仅int I1阳性组对阿米卡星的耐药显著高于阴性组(χ2=5.194,P=0.023)。结论鲍曼不动杆菌多重耐药情况严峻,且生物膜形成能力较强,随着生物膜形成能力的增强其耐药性降低;Bap、bfs、int I1基因参与生物膜形成。
[Abstract]:Objective to investigate the biofilm forming ability of Acinetobacter baumannii clinical isolates and the distribution of biofilm related genes, and to analyze the relationship between biofilm related genes and bacterial drug resistance phenotype. Methods A prospective study was conducted to collect 70 strains of Acinetobacter baumannii isolated from October 2012 to October 2013 in Chengwu County people's Hospital. The biofilm model was constructed with 96 hole polystyrene plates. Quantitative analysis of biofilm forming ability and comparison of antimicrobial resistance of Acinetobacter baumannii with different biofilm formation ability. Polymerase chain reaction (PCR) technique was used to amplify and detect the expression of biofilm-associated protein Bap, bfs and int I1. Results among the 70 Acinetobacter baumannii strains, 40 were multidrug resistant and 6 were pan-resistant. 68 strains were pan-resistant. Among them, 14 were weakly positive, 20 were positive and 34 were strongly positive. The drug resistance rates of Acinetobacter baumannii to imipenem, amikacin, meropenem and cefepime were 30.0032. 86% and 41. 43%, respectively. The resistance rates of Acinetobacter baumannii to imipenem, amikacin, meropenem and cefepime were not less than 50%. With the enhancement of biofilm forming ability, the resistance of Acinetobacter baumannii to levofloxacin (蠂 ~ 2 + 9.225), cefepime (蠂 ~ (2) 7.222) and gentamicin (蠂 ~ (2) 6.601) decreased significantly. The positive rates of Bap gene of Acinetobacter baumannii were 65.00% and 79.41% respectively (蠂 2 / 4.244P = 0.120) and the positive rates of BFS were 35.71g% and 88.2400% respectively (蠂 213.602P 0.001T I1 positive rates were 42.86% and 91.1800%, respectively). The antimicrobial resistance rate of biofilm-associated gene positive group was higher than that of gene negative group. Only int I1 positive group was significantly more resistant to amikacin than that of negative group (蠂 ~ 2 ~ 2 ~ (5.194). Conclusion the multidrug resistance of Acinetobacter baumannii is severe and the ability of biofilm formation is strong. The drug resistance of Acinetobacter baumannii is decreased with the increase of biofilm forming ability.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.5

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