E试验法在淋球菌药物敏感性试验中的应用评价

发布时间：2018-05-20 15:18

本文选题：淋病奈瑟菌 + 琼脂稀释法　；参考：《中国艾滋病性病》2016年12期

【摘要】：目的评价E试验(E-test)法检测淋球菌对5种药物敏感性的临床价值。方法对2014年深圳市淋球菌耐药监测项目收集的100株该地区淋球菌临床分离株,分别采用琼脂稀释法、E-test以及纸片扩散法(K-B法)进行头孢曲松、青霉素、大观霉素、环丙沙星以及阿奇霉素药敏试验,以琼脂稀释法结果作为标准,评价E-test与琼脂稀释法结果的一致率,比较E-test与K-B法的误差率及相关性。结果 E-test与琼脂稀释法测得的100株淋球菌对五种抗生素的50%最小抑菌浓度MIC50、MIC90以及MIC值范围大多完全相同或仅相差0.5~1.5个稀释度,两种方法具有较高的基本一致率(72%~98%),其中一致率最高的是环丙沙星(98%),其次是阿奇霉素(92%),一致率最低的是青霉素(72%)。除青霉素E-test误差率(22%)高于K-B法(12%)外,其余四种抗生素E-test的误差率均低于K-B法,且与琼脂稀释法的相关性高于K-B法。结论环丙沙星和阿奇霉素的E-test可以作为琼脂稀释法的替代方法应用于临床,头孢曲松和大观霉素的E-test可能导致结果偏低,而青霉素由于同时具有较低的一致率和较高的误差率,则不适宜采用。
[Abstract]:Objective to evaluate the clinical value of E-test-E-test in the detection of gonococcal susceptibility to 5 drugs. Methods 100 clinical isolates of Neisseria gonorrhoeae collected from Shenzhen City in 2014 were treated with ceftriaxone penicillin and spectinomycin respectively by Agar dilution method E-test and disk diffusion method (K-B method). Ciprofloxacin and azithromycin susceptibility tests were used to evaluate the consistency between the results of E-test and Agar dilution, and to compare the error rate and correlation between E-test and K-B. Results the minimum inhibitory concentration of 50% MIC50 MIC90 and the range of MIC values of 100 strains of Neisseria gonorrhoeae determined by E-test and Agar dilution method were the same or only 0.5 ~ 1.5 dilution degrees different. The two methods had a higher basic consistency rate of 72X and 98cm, of which the highest was ciprofloxacin, the second was azithromycin, and the lowest was penicillin. Except penicillin E-test error rate (22%) was higher than K-B method (12%), the other four antibiotics E-test error rate was lower than K-B method, and the correlation with Agar dilution method was higher than K-B method. Conclusion the E-test of ciprofloxacin and azithromycin can be used as an alternative to Agar dilution. The E-test of ceftriaxone and spectinomycin may lead to low results, while penicillin has lower consistency and higher error rate. It is not suitable for adoption.
【作者单位】：深圳市慢性病防治中心;
【基金】：深圳市卫生计生系统科研项目(201401071)
【分类号】：R446.5

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