广泛耐药鲍曼不动杆菌ST238克隆株毒力及适应性特征

发布时间：2018-05-31 01:42

本文选题：广泛耐药 + 鲍曼不动杆菌　；参考：《中国感染与化疗杂志》2016年06期

【摘要】：目的通过检测广泛耐药鲍曼不动杆菌流行克隆ST238的毒カ和环境适应性,探讨该克隆株在当地持续流行的机制。方法比较ST238克隆株与鲍曼不动杆菌ATCC 19606的体外生长曲线及竞争指数(competition index.Cl),试管法测定菌株内明胶酶活性;微量法进行D-甘露糖抵抗红细胞凝集试验;运动平板法测定蹭行运动能力;结晶紫染色法定量分析生物膜形成能力。小鼠动物模型检测体内致病力。结果ST238克隆株与标准株间体外生长能力差异无统计学意义(P0.05)。培养24h时ST238克隆株与鲍曼不动杆菌ATCC 19606在体外竞争能力无明显差异(CI=0.29,P0.05)0明胶酶活性在ST238克隆株与敏感株间分布无差异(P0.05)。血凝结果与菌株来源之间差异无统计学意义(P0.05)。敏感株的运动能力及生物膜形成能力均强于ST238克隆株(P0.05)。ST238克隆株与标准株的LD_(50)。分别为6.9和6.7 log CFU/mL。接种9.3 log CFU/mL菌液的小鼠平均死亡时间差异无统计学意义(P0.05)。结论ST238克隆株获得广泛耐药性后,其环境适应性无明显下降,同时该克隆株的毒力也无明显改变。这种生物学特性可能是该克隆株在当地长期流行的原因之一。
[Abstract]:Objective to detect the virulence and environmental adaptability of ST238 clone of Acinetobacter baumannii, and to explore the mechanism of its continued prevalence. Methods the growth curve and competition index of ST238 clone strain were compared with that of Acinetobacter baumannii ATCC 19606 in vitro. The movement ability was measured by exercise plate method and the biofilm formation ability was quantitatively analyzed by crystal violet staining method. The pathogenicity in vivo was tested by mouse animal model. Results there was no significant difference in growth ability between ST238 clone and standard strain in vitro. There was no significant difference in competitive ability between ST238 clone strain and Acinetobacter baumannii ATCC 19606 strain in vitro. There was no difference in the distribution of gelatinase activity between ST238 clone strain and sensitive strain. There was no significant difference between the results of hemagglutination and the source of the strain (P0.05). The motor ability and biofilm forming ability of sensitive strain were better than that of ST238 clone strain P0.05N. ST238 clone strain and standard strain. 6. 9 and 6. 7 log CFU / mL. respectively. There was no significant difference in the mean death time of mice inoculated with 9.3 log CFU/mL solution (P 0.05). Conclusion the environmental adaptability and virulence of ST238 clone were not significantly decreased and the virulence of the clone was not changed after obtaining extensive drug resistance. This biological characteristic may be one of the reasons for the long-term prevalence of the clone.
【作者单位】：重庆医科大学附属第一医院感染科;
【基金】：重庆市自然科学基金项目(CSTC2008BB5393) 重庆市教育委员会科技项目(KJl00311)
【分类号】：R446.5

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