细胞DNA定量分析在颈部淋巴结和浆膜腔积液中的诊断价值

发布时间：2018-06-03 16:23

本文选题：传统制片技术 + 细胞DNA定量分析　；参考：《安徽医科大学》2015年硕士论文

【摘要】：目的:比较细胞DNA定量分析(DNA image cytometry,DNA-ICM)和传统制片技术(Conventional Smears,CS)在颈部淋巴结和浆膜腔积液中的诊断价值。方法:利用细胞DNA定量分析技术和传统制片技术对209例颈部淋巴结穿刺标本和341例浆膜腔积液标本进行比较分析。结果:209例淋巴结穿刺标本中,CS的敏感度、特异度和准确度分别为90%、98.4%及95.2%,而DNA-ICM敏感度、特异度和准确度分别为95%、100%及98.1%。统计学显示两种方法的敏感度、特异度和准确度均无显著差异(P0.05);341例浆膜腔积液标本中,CS的敏感度、特异度和准确度分别为82.8%、93.7%及83.0%,DNA-ICM的敏感度、特异度和准确度分别为88.1%、100%及94.7%,统计学显示两种方法的敏感度无显著差异,而特异度和准确度均有显著差异,可见DNA-ICM在浆膜腔积液的诊断中优于CS,且两种方法联合诊断可以提高诊断的敏感度。结论:DNA-ICM在淋巴结针吸标本的诊断中不优于传统制片技术,并不能提高诊断的敏感度、准确度及特异度;而在浆膜腔积液的诊断中,DNA-ICM有明显优势,诊断的准确度及特异度均高于传统制片技术。
[Abstract]:Objective: to compare the diagnostic value of cellular DNA quantitative analysis of image cystometry DNA-ICM with conventional technique in cervical lymph node and serous cavity effusion. Methods: two hundred and nine cervical lymph node puncture specimens and 341 serous cavity effusion specimens were analyzed by cell DNA quantitative analysis and conventional film making techniques. Results the sensitivity, specificity and accuracy of CS were 9098.4% and 95.22.The sensitivity, specificity and accuracy of DNA-ICM were 95%, 100% and 98.1%, respectively. There was no significant difference in sensitivity, specificity and accuracy between the two methods. The sensitivity, specificity and accuracy of CS in 341 cases of serous effusion were 82.8% and 83.0%, respectively. The specificity and accuracy were 88. 1% and 94. 7%, respectively. There was no significant difference in sensitivity between the two methods, but there were significant differences in specificity and accuracy between the two methods. Therefore, DNA-ICM is superior to CSS in the diagnosis of serous effusion, and the combination of two methods can improve the sensitivity of diagnosis. Conclusion in the diagnosis of lymph node aspiration specimens, the sensitivity, accuracy and specificity of the diagnosis can not be improved by using DNA ICM, but it has obvious advantages in the diagnosis of serous cavity effusion, and it can not improve the sensitivity, accuracy and specificity of the diagnosis of lymph node aspiration specimen, but it has obvious advantages in the diagnosis of serous cavity effusion. The accuracy and specificity of diagnosis were higher than that of traditional technique.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.8

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