脐血间充质干细胞移植对心肌细胞凋亡的影响

发布时间：2018-06-09 05:26

本文选题：脐血干细胞移植 + 肌细胞　；参考：《中国组织工程研究》2016年14期

【摘要】：背景:研究发现,脐血间充质干细胞移植可为减少心肌细胞凋亡、减轻心肌纤维化、改善心脏功能发挥作用。目的:观察脐血间充质干细胞移植对大鼠心肌细胞凋亡的影响。方法:(1)体外实验:将原代培养72 h的正常心肌细胞作为对照组,阿霉素损伤心肌细胞作为损伤组,阿霉素损伤心肌细胞与脐血间充质干细胞共培养作为共培养组,培养48 h采用TUNEL染色评估心肌细胞的凋亡情况;(2)体内实验:SD大鼠45只,随机分为正常对照组、阿霉素组、脐血间充质干细胞移植组,移植后2周采用Powerlab检查心功能,蛋白质免疫印迹法检测凋亡相关蛋白Bax、Caspase-3表达水平,组织学观察各组心肌病理变化。结果与结论:(1)正常对照组的心肌细胞凋亡率较低,损伤组心肌细胞凋亡明显增加,共培养组凋亡率显著降低,各组间比较差异有显著性意义(P0.05);(2)与阿霉素组比较,脐血间充质干细胞移植组的心功能指标明显降低,差异有显著性意义(P0.05),但仍高于正常对照组(P0.05);(3)阿霉素组心肌细胞损伤后Bax、Caspase-3表达水平较高,脐血间充质干细胞移植组Bax、Caspase-3表达水平降低(P0.05),但仍高于正常对照组(P0.05);(4)与阿霉素组比较,脐血间充质干细胞移植组心肌梗死面积显著减小(P0.05),但仍高于正常对照组(P0.05);(5)结果表明,脐血间充质干细胞作用能够抑制阿霉素诱导的大鼠心肌细胞的凋亡,其发挥作用的途径可能与下调Bax、Caspase-3的表达水平有关。
[Abstract]:Background: cord blood mesenchymal stem cell transplantation has been shown to play an important role in reducing cardiomyocyte apoptosis, alleviating myocardial fibrosis and improving cardiac function. Aim: to observe the effect of umbilical cord blood mesenchymal stem cell transplantation on cardiomyocyte apoptosis in rats. Methods: in vitro, normal cardiomyocytes cultured for 72 hours were used as control group, cardiomyocytes injured by adriamycin as injury group and co-cultured with mesenchymal stem cells from umbilical cord blood as co-culture group. Forty-five SD rats were randomly divided into normal control group, adriamycin group and umbilical cord blood mesenchymal stem cell transplantation group. The cardiac function was examined by Powerlab 2 weeks after transplantation. The expression of apoptosis-related protein Baxfen Caspase-3 was detected by Western blot, and the pathological changes of myocardium were observed by histology. Results and conclusion the apoptotic rate of cardiomyocytes in the normal control group was lower than that in the injured group, and the apoptosis rate in the co-cultured group was significantly lower than that in the injured group. The difference between the two groups was significant (P 0.05) and that in the adriamycin group was higher than that in the adriamycin group. The cardiac function index of umbilical cord blood mesenchymal stem cell transplantation group was significantly lower than that of the normal control group (P 0.05, P 0.05, P 0.05). The expression of Baxon Caspase-3 in adriamycin group was higher than that in adriamycin group. The expression of Caspase-3 in umbilical cord blood mesenchymal stem cell transplantation group was significantly lower than that in the normal control group (P 0.05, but still higher than that in the normal control group, P 0.05, P 0.05). Compared with the adriamycin group, the myocardial infarction area of the umbilical cord blood mesenchymal stem cell transplantation group was significantly reduced, but still higher than that of the normal control group. Cord blood mesenchymal stem cells can inhibit the apoptosis of rat cardiomyocytes induced by doxorubicin, and the pathway of its action may be related to the down-regulation of the expression of Baxon Caspase-3.
【作者单位】：唐山市开滦总医院心内科;唐山市遵化市人民医院;
【分类号】：R457.7

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