多尼培南抗铜绿假单胞菌生物被膜的体外活性研究

发布时间：2018-06-10 09:17

本文选题：铜绿假单胞菌 + 生物被膜　；参考：《中国抗生素杂志》2016年06期

【摘要】：目的探究新型碳青霉烯类抗生素多尼培南(doripenem,DOR)抗临床铜绿假单胞菌生物被膜的活性并进一步探究亚抑菌浓度多尼培南抑制生物被膜的作用机制。方法采用微孔板法建立临床铜绿假单胞菌生物被膜体外模型。微量肉汤稀释法测定多尼培南对铜绿假单胞菌的最低抑菌浓度(minimal inhibitory concentration,MIC)和最低抑制生物被膜浓度(minimal biofi lm inhibitory concentration,MBIC)。结晶紫染色法测定生物被膜的形成和黏附,活菌计数法测定生物被膜内菌数。平板分析法测定运动性,微生物碳氢吸附能力(microbial adhesion to hydrocarbons,MATH)测定评估细胞疏水性。结果多尼培南具有较强的抗铜绿假单胞菌浮游菌和生物被膜菌活性,MIC50为0.5μg/m L,MBIC50为2μg/m L。明显抑制生物被膜的形成和减少已形成生物被膜的量,降低铜绿假单胞菌的运动性和疏水性。结论多尼培南能有效抑制铜绿假单胞菌生物被膜,有望用于相关感染的治疗。
[Abstract]:Objective to investigate the anti-biofilm activity of a new carbapenem antibiotic Donipenem doripenememone (DORA) against Pseudomonas aeruginosa and to explore the mechanism of the inhibitory effect of subinhibitory concentration of Donipenem on the biofilm of Pseudomonas aeruginosa (Pseudomonas aeruginosa). Methods A clinical model of Pseudomonas aeruginosa biofilm was established by microporous plate method. Minimal inhibitory concentration and minimal biofi lm inhibitory concentration of Donipenem against Pseudomonas aeruginosa were determined by broth dilution method. The formation and adhesion of biofilm were determined by crystal violet staining and the number of bacteria in biofilm was determined by living bacteria counting method. The hydrophobicity of cells was evaluated by plate analysis and microbial adhesion to hydrocarbonsmath. Results Donipenem had a strong activity against Pseudomonas aeruginosa phytoplankton and biofilm bacteria. The MIC50 was 0.5 渭 g / m ~ (-1) 路m ~ (-1) and 2 渭 g 路m ~ (-1) 路m ~ (-1). The formation of biofilm was inhibited and the amount of biofilm was reduced, and the mobility and hydrophobicity of Pseudomonas aeruginosa were decreased. Conclusion Donipenem can effectively inhibit the biofilm of Pseudomonas aeruginosa and may be used in the treatment of related infections.
【作者单位】：中国药科大学生命科学与技术学院;
【分类号】：R446.5

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