泛耐药肺炎克雷伯菌耐药基因检测及聚类分析

发布时间：2018-06-15 13:40

本文选题：泛耐药 + 肺炎克雷伯菌　；参考：《中华医院感染学杂志》2016年22期

【摘要】：目的调查三家医院31株泛耐药肺炎克雷伯菌(PDR-KPN)获得性耐药相关基因和可移动遗传元件遗传标记的存在状况,并分析其亲缘关系。方法收集三家医院2013年1月-12月分离31株PDR-KPN;采用聚合酶链反应(PCR)法检测β-内酰胺类、氨基糖苷类、喹诺酮类获得性耐药基因以及可移动遗传元件标记基因。对全部耐药基因检测结果作样本聚类分析。结果 31株PDR-KPN每株均检出β-内酰胺类、氨基糖苷类药物获得性耐药基因,喹诺酮类药物作用靶位gyrA基因突变阳性率100%;31株blaKPC与ISKpn6均阳性,blaKPC-ISKpn6连锁检测阳性率100.0%。样本聚类分析显示A、B、C三家医院的菌株分为三个簇群。B医院与C医院的菌株有较近的亲缘关系。结论本组菌株携带获得性耐药相关基因导致对相关抗菌药物耐药。指标聚类分析提示菌株之间存在克隆传播。
[Abstract]:Objective to investigate the existence of acquired resistance related genes and genetic markers of mobile genetic components of 31 drug-resistant Klebsiella pneumoniae (PDR-KPN) in three hospitals, and to analyze their genetic relationship. Methods 31 strains of PDR-KPN were isolated from three hospitals in January 2013 and 31 were isolated from -12 months, and beta lactam and aminoglycosides were detected by polymerase chain reaction (PCR). Quinolones acquired resistance gene and movable genetic element marker gene. The results of all drug resistance genes were analyzed by cluster analysis. Results of 31 PDR-KPN strains, beta lactam, aminoglycoside drug acquired resistance gene, and gyrA gene mutation positive rate of quinolone drug target were 100%, 31 strains of blaKPC and ISKp N6 positive, blaKPC-ISKpn6 linkage detection positive rate 100.0%. sample cluster analysis showed that the strains of three hospitals in A, B, C were divided into three cluster groups.B hospital and C hospital strains have closer relationship. Conclusion this group of strains carrying acquired resistance related genes lead to resistance to related antimicrobial agents. Index cluster analysis suggests that the strains exist among the strains. In cloned transmission.
【作者单位】：苏州大学附属第一医院老年医学科;宁波大学医学院附属医院微生物实验室;宁波大学医学院附属医院呼吸内科;
【基金】：宁波市自然科学基因(2016A610132) 宁波市医学科技基金项目(2011B13)
【分类号】：R446.5

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