南昌市鼠类汉坦病毒检测及基因分型

发布时间：2018-06-17 15:33

本文选题：汉坦病毒检测 + 基因分型　；参考：《现代预防医学》2016年06期

【摘要】：目的了解从江西省南昌市捕捉的鼠类汉坦病毒感染情况,分析汉坦病毒基因型别和基因特征。方法捕鼠取鼠肺,冷冻切片后运用直接免疫荧光法检测鼠肺中汉坦病毒抗原,提取抗原阳性鼠肺标本RNA,采用特异性引物对部分M基因片段进行基因扩增,对扩增产物进行序列测定和基因分析。结果共捕获鼠类130只,褐家鼠(99,76.15%)、小家鼠(13,10.00%)、黄胸鼠(18,13.85%),其中18份汉坦病毒免疫荧光阳性,鼠类汉坦病毒携带率为13.85%,不同地区间汉坦病毒携带率差异有统计学意义(=17.89,P=0.001290.05),青山湖区最高(26.67%);对所有的免疫荧光抗原阳性鼠肺标本进行核酸检测、序列测定和分析,结果发现所有标本汉坦病毒均属汉城型S4亚型,毒株之间核苷酸同源性98.6%~100.0%。结论南昌市鼠间流行的汉坦病毒为汉城型,S4亚型。
[Abstract]:Objective to investigate the infection of rat Hantavirus from Nanchang, Jiangxi Province, and to analyze the gene types and characteristics of Hantavirus. Methods the rat lungs were harvested, and the hantavirus antigens in the lungs were detected by direct immunofluorescence method after frozen sections. The RNAs were extracted from the lungs of the antigen-positive mice, and some M gene fragments were amplified by specific primers. The amplified products were sequenced and analyzed. Results A total of 130 rodents were captured, and Rattus norvegicus was found to be 99.76.150.The mice were 1310.00 and 1813.850.18 of them were positive for Hantavirus immunofluorescence. The carrying rate of rat Hantavirus was 13.85. The difference of Hantavirus carrying rate among different regions was statistically significant, and the highest rate was 26.670.The nucleic acid was detected, sequenced and analyzed in all the lung samples of positive mice with immunofluorescence antigen. The results showed that Hantavirus in all specimens belonged to S4 subtype of Seoul type, and the nucleotide homology was 98.6 and 100.0. Conclusion the Hantavirus epidemic in Nanchang City is a S4 subtype of Seoul type.
【作者单位】：江西省疾病预防控制中心疾病检测所;
【基金】：江西省卫生厅科技计划项目(20123156)
【分类号】：R512.8;R440

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