血浆miRNA-30、miRNA-199、miRNA-208及miRNA-374在ACS诊断中的应用研究

发布时间：2018-06-21 19:25

  本文选题：急性心肌梗死 + miRNA　； 参考：《昆明医科大学》2015年硕士论文

【摘要】：目的：miRNA通过调节基因的表达,广泛参与心血管系统细胞的增殖、迁移、分化、凋亡等病理生理过程,在心血管系统疾病的发生发展过程中起着重要的调控作用。本研究采用实时荧光定量PCR,检测血浆中miRNA-30d、miRNA-199a、 miRNA-208b和miRNA-374b的表达情况,研究在STEMI、NSTEMI、UA、SA及健康对照组中的差异,探讨血浆miRNA作为急性心肌梗死诊断生物学标记物的可能性。方法：收集50位STEMI患者,50位NSTEMI患者,50位UA患者,50位SA患者及30位健康对照组血液,提取总RNA,利用实时荧光定量PCR技术检测miRNA的表达量,并对各组间相关miRNA进行统计学分析。同时检测患者cTnI和CK-MB,分析与目的miRNA的相关性,及在诊断急性心肌梗死中的敏感度和特异性分析。结果：血浆miRNA在STEMI组、NSTEMI组、UA组、SA组中表达升高,明显高于健康对照组,具有统计学意义(P0.05)。miRNA表达水平与cTnI和CK-MB呈正相关。ROC曲线显示miRNA在诊断急性心肌梗死时明显优于cTnI和CK-MB,特别是胸痛发作后3小时内。结论：实验结果提示心肌细胞特异性的miRNA可作为一类新的生物标志物用于急性心肌梗死的早期诊断及治疗评估。
[Abstract]:Objective to regulate the expression of genes and participate in the pathophysiological processes such as proliferation, migration, differentiation and apoptosis of cardiovascular system cells, which play an important role in the development of cardiovascular diseases. In this study, real-time fluorescent quantitative PCRs were used to detect the expression of miRNA-30dnmRNA-199ab, miRNA-208b and miRNA-374b in plasma. The differences were studied in STEMIN NSTEMIMIUASA and healthy controls, and the possibility of using plasma miRNAs as biological markers in the diagnosis of acute myocardial infarction (AMI) was investigated. Methods: 50 patients with STEMI, 50 patients with NSTEMI, 50 patients with UA, 50 patients with SA and 30 healthy controls were collected, the total RNAs were extracted and the expression of miRNA was detected by real-time fluorescence quantitative PCR. The relationship between cTnI and CK-MBs and miRNA, sensitivity and specificity in the diagnosis of acute myocardial infarction were analyzed. Results: the expression of miRNA in plasma was significantly higher in the SA group than that in the control group, and the expression of miRNA in the SA group was significantly higher than that in the healthy control group. The positive correlation between miRNA expression and cTnI and CK-MB showed that miRNA was superior to cTnI and CK-MBin in the diagnosis of acute myocardial infarction, especially within 3 hours after chest pain attack. Conclusion: the results suggest that miRNA can be used as a new biomarker for the early diagnosis and treatment of acute myocardial infarction.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.11

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1 李勤;血浆miRNA-30、miRNA-199、miRNA-208及miRNA-374在ACS诊断中的应用研究[D];昆明医科大学;2015年

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