ICU耐甲氧西林金黄色葡萄球菌分布特征分析

发布时间：2018-06-29 01:45

本文选题：医院感染 + 耐甲氧西林金黄色葡萄球菌　；参考：《中华医院感染学杂志》2016年11期

【摘要】：目的监测医院ICU医务人员手及环境表面中耐甲氧西林金黄色葡萄球菌(MRSA)的污染情况,为医院采取有效合理的感染控制措施提供依据。方法于2012年6月采用无菌棉签对医院ICU的24名医务人员手及高频接触的物品表面进行采样,用MRSA显色培养皿和PCR技术扩增特征基因nuc和mecA的方法分离、鉴定MRSA,并对分离到的菌株采用法国生物梅里埃公司ATB微生物鉴定药敏系统进行耐药性检测,采用SPSS19.0软件进行数据录入和统计。结果医务人员手MRSA检出率为41.7%,其中保洁员检出率最高,为75.0%;共采集环境标本62份,检出MRSA 7株,检出率为11.3%,其中床单元、医疗设备、公共物品的MRSA检出率差异无统计学意义;所有MRSA对青霉素和苯唑西林的耐药率均为100.0%,但是对米诺环素、喹奴普汀/达福普汀、利福平、替考拉宁和万古霉素敏感率均为100.0%。结论 ICU医务人员手MRSA的检出率较高,环境表面存在MRSA污染,医院应加强教育培训,提高医务人员手卫生意识和提高环境清洁质量。
[Abstract]:Objective to monitor the contamination of methicillin-resistant Staphylococcus aureus (MRSA) in hands and environment of ICU medical staff in hospital, and to provide evidence for effective and reasonable infection control measures. Methods Aseptic cotton swabs were used to sample the hands of 24 medical staff and objects in high frequency contact in hospital ICU in June 2012. The characteristic genes nuc and mecA were amplified by nuc and mecA. MRSAs were identified and drug resistance was detected by ATB microorganism identification system of French Biomerier Company. SPSS 19.0 software was used for data entry and statistics. Results the detectable rate of MRSA was 41.7% in the hands of medical staff, among which the detectable rate of cleaning staff was the highest (75.0%), there were 62 samples of environmental samples, 7 strains of MRSA were detected, and the detection rate was 11.3%. There was no significant difference in the detectable rate of MRSA among bed units, medical equipment and public goods. The resistance rates of all MRSA to penicillin and oxacillin were 100.0, but sensitive to minocycline, quinoplatin / dafopentin, rifampicin, teicoplanin and vancomycin were all 100.0. Conclusion the detection rate of MRSA in ICU medical staff is high and MRSA pollution exists on the surface of the environment. Hospitals should strengthen education and training, improve the hand hygiene awareness of medical staff and improve the quality of environmental cleanliness.
【作者单位】：杭州市疾病预防控制中心消毒与病媒生物防制所;
【基金】：浙江省重点科技创新团队基金资助项目(2011R50021) 杭州市科技发展计划基金资助项目(20130733Q32)
【分类号】：R446.5

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