REM期睡眠剥夺对大鼠不同脑区组蛋白H3K9和H3K4甲基化水平的影响

发布时间：2018-06-30 17:40

本文选题：REM期睡眠剥夺 + 组蛋白三甲基化　；参考：《神经解剖学杂志》2016年03期

【摘要】：目的:研究REM期睡眠剥夺对成年大鼠不同脑区包括海马、腹内侧前额叶皮质、下丘脑和中缝核群的组蛋白H3K9和H3K4三甲基化水平的影响。方法:将成年SD大鼠随机分为正常对照组(con)、睡眠剥夺1 d组(SD1 d)、睡眠剥夺3 d组(SD3 d)、睡眠剥夺6 d组(SD6 d)。采用改良式多平台水环境法建立REM期睡眠剥夺模型,分别于睡眠剥夺后1、3、6 d断头取脑,用免疫荧光染色与Western Blot检测大鼠海马、腹内侧前额叶皮质、下丘脑和中缝核群H3K9和H3K4三甲基化水平的变化,最终结果进行统计学分析。结果:Western Blot结果显示:(1)海马、腹内侧前额叶皮质三个SD组H3K9三甲基化水平均低于control组(P0.05),H3K4三甲基化水平均高于control组(P0.05);(2)下丘脑三个SD组H3K9三甲基化水平均低于control组(P0.01),但SD1 d组H3K4三甲基化水平高于control组(P0.05),SD3 d、SD6 d组H3K4三甲基化水平低于control组(P0.05);(3)中缝核群三个SD组H3K9和H3K4三甲基化水平均高于control组(P0.05)。对海马与下丘脑免疫荧光验证H3K9三甲基化结果与Western Blot结果趋势一致,且说明细胞无减少。结论:睡眠剥夺可能与海马、腹内侧前额叶皮质、下丘脑和中缝核群的组蛋白H3K9和H3K4三甲基化水平密切相关。
[Abstract]:Aim: to investigate the effects of REM sleep deprivation on histone H3K9 and H3K4 trimethylation in different brain regions of adult rats, including hippocampus, ventromedial prefrontal cortex, hypothalamus and raphe nuclei. Methods: adult SD rats were randomly divided into normal control group (SD1 d), sleep deprivation for 3 days) (SD3 d), sleep deprivation for 6 days) (SD6 d). The REM sleep deprivation model was established by modified multi-platform water environmental law. The brain was cut off on the 1st and 3rd day after sleep deprivation. The hippocampus and ventromedial prefrontal cortex of rats were detected by immunofluorescence staining and Western Blot. The changes of H _ 3K _ 9 and H _ 3K _ 4 trimethylation levels in hypothalamus and raphe nuclei were statistically analyzed. Results: Western blot: (1) Hippocampal, The level of H3K9 trimethylation in the three SD groups of the ventromedial prefrontal cortex was lower than that in the control group (P0.05). The level of H3K9 trimethylation in the three SD groups of hypothalamus (P0.05); (2) was lower than that in the control group (P0.01), but the level of H3K4 trimethylation in SD1 day group was higher than that in the control group (P0.05). The level of H3K4 trimethylation in control group (P0.05) was lower than that in control group (P0.05); (3). The levels of H3K9 and H3K4 trimethylation in three SD groups of raphe nucleus group were higher than those in control group (P0.05). The results of H3K9 trimethylation in hippocampus and hypothalamus were consistent with those of Western Blot and showed that there was no decrease in H3K9 trimethylation. Conclusion: sleep deprivation may be associated with histone H3K9 and H3K4 trimethylation in hippocampus, ventromedial prefrontal cortex, hypothalamus and raphe nuclei.
【作者单位】：第四军医大学西京医院神经内科;甘肃省碌曲县妇幼保健站;
【分类号】：R740

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