多重PCR检测在严重脓毒症感染患儿应用的临床意义

发布时间：2018-07-02 22:10

本文选题：多重PCR + 严重脓毒症　；参考：《中华医院感染学杂志》2016年14期

【摘要】：目的研究多重PCR检测在严重脓毒症感染患儿中的应用及临床意义,为指导临床治疗提供依据。方法选取2014年10月-2015年10月医院诊治192例严重脓毒症并发感染患儿资料进行分析,患儿入院后进行常规方法检测,利用多重PCR技术定量检测患儿脑脊液EB病毒(EBV)、单纯疱疹病毒(HSV)、巨细胞病毒(CMV)以及肺炎支原体(MP)的核酸含量,并与脑脊液中特异性抗体结果进行比较,分析多重PCR在严重脓毒症并发感染患儿中的应用效果。结果入选192例患儿,84例阳性,阳性率为43.8%,且患儿主要集中在(3~6)岁年龄段;84例患儿脑脊液DNA阳性主要分布在EBV,占46.4%,其次分布HSV,占28.6%;84例患儿中,22例重型,62例轻型,重型患儿EBV-IgM、HSV-IgM、CMV-IgM以及MP-IgM水平,显著高于轻型患儿(P0.05);192例患儿中,45例IgM阳性,145例IgM阴性;患儿中多重PCR检测184例阴性,84例阳性。结论严重脓毒症并发感染患儿采用多重PCR技术临床诊断效果理想,能成为脓毒症患儿常规检测手段,为提高临床诊断效果具有重要意义。
[Abstract]:Objective to study the application and clinical significance of multiplex PCR in children with severe sepsis. Methods data of 192 children with severe sepsis complicated with infection in hospital from October 2014 to October 2015 were analyzed. The nucleic acid contents of EB virus (EBV), herpes simplex virus (HSV), cytomegalovirus (CMV) and mycoplasma pneumoniae (MP) in cerebrospinal fluid (CSF) were detected quantitatively by multiplex PCR, and compared with the results of specific antibodies in CSF. To analyze the effect of multiplex PCR in children with severe sepsis complicated with infection. Results there were 84 cases with positive rate of 43.8% in 192 children, and 84 cases of cerebrospinal fluid DNA were mainly distributed in EBV, accounting for 46.4%, and HSV was the second, accounting for 22 cases of severe or light type of disease in 28.6% of the 84 cases, and the positive rate of cerebrospinal fluid DNA in 84 cases was mainly in EBV, accounting for 46.4%, followed by HSV, accounting for 28.6% of the 84 cases, 22 cases were severe and 62 cases were light. The levels of CMV-IgM and MP-IgM in severe children were significantly higher than those in mild children (P0.05). Conclusion Multiplex PCR is an effective method for the diagnosis of severe sepsis complicated with infection in children. It can be used as a routine method for the detection of sepsis and has great significance in improving the clinical diagnosis.
【作者单位】：兰州大学第二医院小儿重症科;
【基金】：甘肃省科技厅基金资助项目(145RJZA233)
【分类号】：R720.597;R440

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