预先电刺激小脑顶核对大鼠局灶脑缺血溶栓时间窗内、窗外溶栓预后的影响

发布时间：2018-07-12 18:58

本文选题：小脑核 + 电刺激　；参考：《第三军医大学学报》2016年01期

【摘要】：目的探讨预先电刺激小脑顶核(fastigial nucleus stimulation,FNS)启动中枢神经系统内源性神经保护对大鼠局灶脑缺血溶栓时间窗的影响,探讨PPARγ介导炎性机制在FNS对溶栓预后影响中的作用。方法将60只SD大鼠按随机数字表法分成FNS组和对照组,每组30只。FNS组预先给予电刺激小脑顶核1 h,电刺激完成后即刻制备大鼠大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)模型;对照组不予电刺激小脑顶核,仅制备MCAO模型。分别于栓塞后1、3、6、9、12、15 h溶栓;溶栓后72 h予以神经功能评分后处死大鼠,断头取脑,梗死边缘半暗带组织行Western blot和荧光定量PCR(RT-PCR)检测PPARγ表达水平,比色法检测髓过氧化物酶(myeloperoxidase,MPO)活性。结果FNS组能改善神经功能评分,PPARγ表达水平随溶栓再灌注时间呈逐渐增高的趋势,表达高峰可能在梗死后9 h前后,Western blot和RT-PCR检测结果均提示3、6、9、15 h FNS组与其对照组比较差异有统计学意义(P0.05);6、12 h和15 h FNS组脑梗死边缘区MPO活性与对照组比较差异有统计学意义(P0.05)。结论 FNS对脑梗死大鼠有保护作用,并可适当延长大鼠局灶脑缺血溶栓时间窗,该作用可能与PPARγ介导的炎性调控作用有关。
[Abstract]:Objective to investigate the effect of prestimulation of cerebellar parietal nucleus (fastigial nucleus) stimulation on the time window of thrombolytic therapy in rats with focal cerebral ischemia, and to explore the role of (fastigial nucleus 纬 -mediated inflammatory mechanism in the prognosis of thrombolytic therapy. Methods Sixty Sprague-Dawley rats were randomly divided into FNS group and control group. 30 rats in each group were given electrical stimulation of cerebellar parietal nucleus for 1 hour. The model of middle cerebral artery occlusion (MCAO) was established immediately after electrical stimulation. In the control group, no electrical stimulation of cerebellar parietal nucleus was given, only the MCAO model was prepared. The rats were killed at 72 h after thrombolytic thrombolysis for 15 h after thrombolysis, and the brain was cut off. The expression level of PPAR- 纬 was detected by Western blot and fluorescence quantitative polymerase chain reaction (RT-PCR). The activity of myeloperoxidase (MPO) was detected by colorimetric method. Results in FNS group, the expression of PPAR 纬 increased with the time of thrombolytic reperfusion. The results of Western blot and RT-PCR before and after 9 h after infarction showed that there was significant difference between FNS group and control group at 15 h after infarction (P0.05). (P0.05) the activity of MPO in the marginal area of cerebral infarction in FNS group was significantly higher than that in control group at 12 h and 15 h after infarction (P0.05). Conclusion FNS has protective effect on rats with cerebral infarction and can prolong the thrombolytic time window of focal cerebral ischemia in rats, which may be related to the inflammatory regulation mediated by PPAR 纬.
【作者单位】：第三军医大学新桥医院神经内科;重庆医科大学第一附属医院神经内科;第三军医大学新桥医院放射科;
【基金】：重庆自然科学基金重点项目(CSTC2011BA5012) 国家自然科学基金面上项目(81171113)~~
【分类号】：R454.1;R743.3

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