患儿全血淋巴细胞及血清中EB病毒核酸定量结果的比较研究

发布时间：2018-08-03 07:20

【摘要】：目的比较EB病毒(EBV)感染患儿全血淋巴细胞和血清中EB病毒DNA(EBV-DNA)定量分析结果的关系,探讨如何根据患儿感染状态合理选择临床标本类型。方法收集医院2015年9月-12月疑似EB病毒感染的患儿199例,分别取患儿的外周全血及血清,采用实时荧光PCR定量检测EBV-DNA及酶联免疫吸附法测定EBV-CA-IgM,数据采用SPSS 13.0软件进行统计分析。结果全血淋巴细胞EBV-DNA阳性标本95例,阳性率为47.7%;血清EBV-DNA阳性标本36例,阳性率为18.1%;血清EBV-CA-IgM抗体阳性23例,阳性率为11.6%;当血清IgM抗体阴性时,两种标本类型EBV-DNA阳性率差异有统计学意义(P0.01);将全血及血清EBV-DNA均为阳性的标本进行配对分析,定量结果呈正相关(P0.01)。结论全血中淋巴细胞的EBV-DNA阳性率及拷贝数均高于血清。临床上可以根据患儿的病情来选择合适的标本。
[Abstract]:Objective to compare the relationship between the quantitative analysis of EBV DNA (EBV-DNA) in whole blood lymphocytes and serum of children with EBV (EBV) infection and to explore how to select the clinical specimens according to the infective status of EBV in children with Epstein-Barr virus (EBV) infection. Methods 199 children with suspected Epstein-Barr virus infection from September to December 2015 were collected and their peripheral blood and serum were collected respectively. EBV-DNA and EBV-CA-IgM were quantitatively detected by real-time fluorescence PCR and enzyme linked immunosorbent assay (Elisa). The data were analyzed by SPSS 13.0 software. Results the positive rate of EBV-DNA in whole blood lymphocytes was 47.7%, the positive rate of serum EBV-DNA was 18.1%, the positive rate of EBV-CA-IgM antibody was 11.6% in 23 cases, and the positive rate was 11.6% when the serum IgM antibody was negative. The positive rate of EBV-DNA was significantly different between the two types of samples (P0.01), and the positive rate of EBV-DNA in whole blood and serum samples was analyzed by paired analysis, and the quantitative results were positively correlated (P0.01). Conclusion the positive rate and copy number of EBV-DNA in whole blood lymphocytes are higher than those in serum. The appropriate specimen can be selected clinically according to the patient's condition.
【作者单位】：武汉市妇女儿童医疗保健中心检验部;
【基金】：国家自然科学基金资助项目(81201604) 湖北省自然科学基金资助项目(2012FFB05301)
【分类号】：R725.1;R440

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