流式细胞术检测红细胞渗透脆性试验方法的建立

发布时间：2018-08-04 08:05

【摘要】：目的:建立流式细胞术检测红细胞渗透脆性的方法。方法:将红细胞悬浮于由Nacl液和去离子水制备的不同浓度低渗盐溶液(0.70 ml生理盐水+0.3 ml去离子水,0.60 ml生理盐水+0.40 ml去离子水和0.55 ml生理盐水+0.45 ml去离子水)中,利用流式细胞仪及时测定残留红细胞数量。结果:3种NaCl稀释浓度中,流式细胞仪检测的不同时间区域剩余红细胞数量百分比差别不大。选取B+C+D+E+F+G区域比较,不同NaCl稀释浓度下3组的剩余红细胞数量百分比均有差异;正常对照组的剩余红细胞百分比显著低于血红蛋白病组;遗传性球形红细胞增多症(hereditary spherocytosis,HS)组显著低于血红蛋白病组和正常组(P0.05)。3种不同稀释浓度比较发现第2种浓度(0.60 ml N.S.+0.40 ml H_2O)更适合于本实验室Beckman-Coulter流式细胞仪FC500对遗传性球形红细胞增多症的筛查。结论:选用流式细胞术检测红细胞渗透脆性简单、快速、客观、经济。本方法可能是一种非常有效的对遗传性球形红细胞进行初筛的方法。
[Abstract]:Objective: to establish a flow cytometry method for detecting osmotic fragility of erythrocytes. Methods: erythrocytes were suspended in different concentrations of hypotonic salt solution (0.70 ml saline 0.3 ml deionized water 0.60 ml normal saline 0.40 ml deionized water and 0.55 ml normal saline 0.45 ml deionized water) prepared from Nacl solution and deionized water. Flow cytometry was used to determine the number of residual red blood cells in time. Results among the three concentrations of NaCl dilution, the percentage of residual red blood cells detected by flow cytometry was not different in different time regions. The percentage of residual red blood cells in the three groups was significantly lower than that in the hemoglobinopathy group, and the percentage of residual red blood cells in the normal control group was significantly lower than that in the hemoglobin disease group. Compared with hemoglobin group and normal group (P0.05), the second concentration (0.60ml N.S.0.40ml H2O) was more suitable for Beckman-Coulter flow cytometry (FC500) in our laboratory than that of hemoglobin disease group (P0.05) and normal group (P0.05). The results showed that the second concentration (0.60ml N.S0.40ml H2O) was more suitable for genetic analysis. Screening for spheroid polycythemia. Conclusion: using flow cytometry to detect erythrocyte osmotic fragility is simple, rapid, objective and economical. This method may be a very effective method for screening hereditary spherical erythrocytes.
【作者单位】：昆明理工大学附属医院检验科;昆明理工大学附属医院神经内科;云南省第三人民医院医学检验科;云南省肿瘤医院检验科;
【分类号】：R556.6;R446.11

【相似文献】