四川动物源大肠杆菌耐药性、产β-内酰胺酶及血清型的检测

发布时间：2018-08-04 17:36

【摘要】：目的为了解四川地区动物源大肠杆菌临床分离菌株耐药性,产β-内酰胺酶以及产酶菌株血清型流行规律。方法采用微量肉汤稀释法对467株临床菌株进行了27种抗菌药物的耐药性检测;采用CLSI推荐产ESBLs、AmpC和碳青霉烯酶检测方法检测了所有菌株产β-内酰胺酶情况;采用O抗原鉴定法对产酶菌株血清型进行了鉴定。结果 467株菌对27种抗生素产生了不同程度耐药性;菌株产ESBLs、AmpC和碳青霉烯酶检出率分别为36.83%、13.70%和0.00%,产酶菌比非产酶菌具有更严重的耐药性;176株产酶菌共检测出31种血清型,以O131、O107、O78、O9、O127、O20和O157为优势血清型。结论本次检测大肠杆菌ESBLs和AmpC检出率较高,耐药形势严重,血清型复杂,应加强动物源大肠杆菌产β-内酰胺酶及血清型的监测。
[Abstract]:Objective to investigate the drug resistance, 尾-lactamase production and serotype prevalence of Escherichia coli strains isolated from animals in Sichuan province. Methods the drug resistance of 27 antimicrobial agents in 467 clinical strains was detected by broth dilution method, and 尾 -lactamase production of all strains was detected by CLSI recommended production of ESBLsAmpC and carbapenem. The serotype of the enzyme producing strain was identified by O antigen identification method. Results 467 strains were resistant to 27 kinds of antibiotics to varying degrees, and the detection rates of ESBLsAmpC and carbapenem were 36.83% and 0.000%, respectively. A total of 31 serotypes were detected in 176 strains of enzyme producing bacteria with more serious drug resistance than non-enzyme producing bacteria. The dominant serotypes were O 131, O 107, O 78, O 9, O 127, O 20 and O 157. Conclusion the detection rate of ESBLs and AmpC in Escherichia coli is high, the situation of drug resistance is serious, and the serotype is complex. The surveillance of 尾 -lactamases and serotypes in E. coli should be strengthened.
【作者单位】：四川农业大学动物医学院新药安全评价实验室;四川农业大学动物医学院药物制剂实验室;
【基金】：国家科技支撑计划(No.2012BAC01B06)~~
【分类号】：R446.5

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