川芎嗪对内毒素性休克大鼠脑损伤的作用

发布时间：2018-08-10 20:05

【摘要】：目的:研究川芎嗪(ligustrazine,Lig)对内毒素性休克大鼠脑损伤的作用并探讨其作用机制。方法:将48只健康Wistar大鼠随机分为正常组、LPS组和LPS+Lig组,每组16只,上述每组再分为2个亚组:6 h组和12 h组,各8只大鼠。以尾静脉注射5 mg/kg脂多糖(LPS)建立内毒素性休克大鼠模型,腹腔注射盐酸川芎嗪注射液200 mg/kg,分别在相应时点进行眼球摘除采血并断头取脑组织,ELISA法检测血清中神经元特异性烯醇化酶(neuron-specific enolase,NSE)含量,脑组织匀浆检测一氧化氮(nitric oxide,NO)含量,TUNEL染色法检测脑组织中海马区细胞凋亡情况,Western blot法检测Bax和Bcl-2蛋白的表达。结果:与LPS组相比,川芎嗪能降低内毒素性休克所致大鼠NSE和NO含量的上升,同时抑制海马区细胞的凋亡,增加Bax的蛋白表达并降低Bcl-2的蛋白表达。结论:川芎嗪通过降低NSE和NO含量,减轻内毒素性休克大鼠的脑损伤程度,可能与其调节Bax和Bcl-2蛋白的表达有关。
[Abstract]:Aim: to study the effect of ligustrazine Lig on brain injury in rats with endotoxic shock and its mechanism. Methods: Forty-eight healthy Wistar rats were randomly divided into normal group (n = 16) and LPS Lig group (n = 16). Each group was divided into 2 subgroups: 1: 6 h group and 12 h group, 8 rats in each group. Lipopolysaccharide (LPS) was injected into tail vein for 5 mg/kg to establish the rat model of endotoxic shock. Intraperitoneal injection of ligustrazine hydrochloride injection was performed at 200 mg / kg. Blood was taken from eyeball at the corresponding time point and brain tissue was taken from head off to detect the content of neuron-specific enolase in serum by Elisa. The content of nitric oxide (no) in brain homogenate and the expression of Bax and Bcl-2 protein in hippocampus were detected by Tunel staining and Western blot. Results: compared with LPS group, ligustrazine could decrease the contents of NSE and no, inhibit the apoptosis of hippocampal cells, increase the protein expression of Bax and decrease the protein expression of Bcl-2. Conclusion: Ligustrazine can decrease the level of NSE and no and reduce the degree of brain injury in rats with endotoxic shock, which may be related to its regulation of the expression of Bax and Bcl-2 proteins.
【作者单位】：河南省新乡市中心医院神经内科;
【分类号】：R459.7

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