我国部分地区献血人群中单纯疱疹病毒感染流行情况
发布时间：2018-08-20 13:14
【摘要】：目的了解中国献血人群中单纯疱疹病毒(HSV)的流行率、病毒载量及抗体检出情况。方法构建的实时荧光定量PCR体系,检测来自重庆、洛阳、乌鲁木齐、绵阳和柳州等5个采供血机构3 794(人)份献血者血浆标本的HSV DNA,并测定DNA阳性标本的病毒载量;同时随机抽取其中292(人)份标本用ELISA法检测Ig G和Ig M抗体的感染情况。结果在本组献血者人群中,发现43份HSV DNA反应性标本,占1.13%(43/3 794),5个采供血机构的检出率分别为重庆1.24%(20/1611)、洛阳0(0/624)、乌鲁木齐3.63%(14/413)、绵阳1.88%(9/479)和柳州0(0/667);病毒载量(9.15×102-9.65×104)(中位数3.53×103)cp/m L。292份经ELISA检测的标本中,Ig G抗体阳性率16.44%(48/292),Ig M抗体阳性率4.45%(13/292),Ig G、Ig M抗体双阳性率2.40%(7/292)。结论目前HSV在我国献血人群中存在一定的流行率,但是检测出的HSV DNA阳性标本的病毒载量较低;是否需要对特殊受血者做血液HSV检测值得进一步探讨。
[Abstract]:Objective To investigate the prevalence, viral load and antibody detection of herpes simplex virus (HSV) in Chinese blood donors. Methods A real-time fluorescence quantitative PCR system was established to detect the HSV DNA in 3 794 blood donors from 5 blood donation institutions including Chongqing, Luoyang, Urumqi, Mianyang and Liuzhou, and to detect the disease of DNA positive samples. Results 43 HSV DNA reactive specimens (43/3 794) were found in this group of blood donors. The detection rates of 5 blood donors were 1.24% (20/1611) in Chongqing, 0 (0/624) in Luoyang, 3.63% (14/413) in Urumqi, 1.8% in Mianyang and 3.13% (14/413) in Urumqi, respectively. 8% (9/479) and Liuzhou 0 (0/667); viral load (9.15 *102-9.65 *104) (median 3.53 *103) cp/m L.292 samples tested by ELISA, the positive rate of Ig G antibody was 16.44% (48/292), the positive rate of Ig M antibody was 4.45% (13/292), the double positive rate of Ig G and Ig M antibody was 2.40% (7/292). Conclusion There was a certain prevalence of HSV in Chinese blood donors at present. The viral load of HSV DNA positive specimens was low, and whether it was necessary to detect HSV in blood of special recipients should be further explored.
【作者单位】： 中国医学科学院北京协和医学院输血研究所;
【分类号】：R440
[Abstract]:Objective To investigate the prevalence, viral load and antibody detection of herpes simplex virus (HSV) in Chinese blood donors. Methods A real-time fluorescence quantitative PCR system was established to detect the HSV DNA in 3 794 blood donors from 5 blood donation institutions including Chongqing, Luoyang, Urumqi, Mianyang and Liuzhou, and to detect the disease of DNA positive samples. Results 43 HSV DNA reactive specimens (43/3 794) were found in this group of blood donors. The detection rates of 5 blood donors were 1.24% (20/1611) in Chongqing, 0 (0/624) in Luoyang, 3.63% (14/413) in Urumqi, 1.8% in Mianyang and 3.13% (14/413) in Urumqi, respectively. 8% (9/479) and Liuzhou 0 (0/667); viral load (9.15 *102-9.65 *104) (median 3.53 *103) cp/m L.292 samples tested by ELISA, the positive rate of Ig G antibody was 16.44% (48/292), the positive rate of Ig M antibody was 4.45% (13/292), the double positive rate of Ig G and Ig M antibody was 2.40% (7/292). Conclusion There was a certain prevalence of HSV in Chinese blood donors at present. The viral load of HSV DNA positive specimens was low, and whether it was necessary to detect HSV in blood of special recipients should be further explored.
【作者单位】： 中国医学科学院北京协和医学院输血研究所;
【分类号】：R440
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