番禺地区ABO亚型基因分型研究

发布时间：2018-08-20 18:18

【摘要】：目的对广州番禺地区人群开展ABO亚型筛查和基因分型研究,了解该地区ABO亚型的分布频率和基因分型的特征。方法随机抽取本地区参加献血和临床体检样本,采用96孔微量板法快速筛查ABO血型正定型和反定型,凡是正反定型不一致的进一步做亚型鉴定;采用抗-A_1试剂对经单克隆ABO正定型试剂确定为A型和AB型的样本进行A_2和A_2B亚型筛查,采用试管法进行血清学鉴定;采用PCR-SSP法对上述鉴定为亚型样本的进行基因分型,对特殊样本进行测序分析。结果在39 106例ABO正反定型检测中,检出2例A_3亚型、基因分型均为A102/O01,1例B_3亚型、基因分型B303/O02。在6 664例经单克隆试剂定型A型中筛检出16例A_2亚型,发生频率约为0.24%(16/6 664);基因分型4例A205和2例A201,发生频率约为0.09%(6/6 664)。在834例经单克隆试剂定型AB型中筛检出44例A_2B亚型,发生频率约为5.28%(44/834),基因分型检出15例A205/B01、4例A201/B01,发生频率约为2.28%(19/834)。结论本地区人群中发现A_2、A_2B、A_3、B_3等亚型,使用PCR-SSP方法结合血清学检测更能准确鉴定ABO亚型。
[Abstract]:Objective to investigate the distribution frequency and genotyping of ABO subtypes in Panyu area of Guangzhou. Methods the blood donation and clinical physical examination samples were randomly selected, and the positive and negative ABO blood group types were quickly screened by 96 well microplate method, and the subtypes were further identified if the positive and negative blood types were inconsistent. The samples identified as type A and AB by monoclonal ABO positive reagents were screened for Astu2 and A2B subtypes, serological identification was carried out by test tube method, and genotyping of the samples identified as subtypes was carried out by PCR-SSP method. The special samples were sequenced and analyzed. Results among 39 106 cases of ABO positive and negative typing, 2 cases were detected as subtypes of ACS 3, all of which were genotyped as A102 / O01C, and 1 of them was subtype B 3. The genotyping was B303 / O02. Among the 6 664 cases identified by monoclonal reagent, 16 cases of ASC2 subtype were detected with frequency of 0.24% (16 / 6664) and 4 cases of A205 and 2 cases of A201 with a frequency of 0.09% (6 / 6664). In 834 cases of AB type identified by monoclonal reagents, 44 cases were screened for A2B subtype, the frequency of occurrence was about 5.28% (44 / 834), and 15 cases (A205 / B01 / 4, A201 / B01) were detected by genotyping, the frequency of occurrence was about 2.28% (19 / 834). Conclusion the subtypes of Astand _ 2C _ 2B _ (2) C _ (2) C _ (2) B _ (3) were found in the population in this area. The ABO subtype could be identified more accurately by using PCR-SSP method combined with serological detection.
【作者单位】：广州市番禺区中心血站;
【基金】：广州市番禺区科技和信息化局2013年医疗卫生计划项目(2013-Z03-64)
【分类号】：R440

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