抑菌试验中抑菌圈法的比较研究

发布时间：2018-08-31 10:52

【摘要】：试验比较研究了抑菌圈法中的K-B法、牛津杯法和打孔法在抑菌试验中的优缺点,结果表明:打孔法操作简单、方便快捷,获得的抑菌圈质量高、重复性好,是优先选用的方法;牛津杯法获得的抑菌圈质量较高,重复性较好;K-B法则只适宜进行定性试验。在进行抑菌圈法试验时,建议使用预加菌液倾注平板法来制备试验平板,试验平板中菌体浓度应在1×10~5~1×10~6 CFU/m L,试验药物应选择适宜的浓度,加入试验药物后应在4℃条件下预扩散2h左右再放置培养箱进行培养,另外还应考虑培养基的pH。
[Abstract]:The advantages and disadvantages of K-B method, Oxford Cup method and perforation method in bacteriostasis test were compared. The results showed that the method was simple, convenient and quick, the quality of the circle was high and the reproducibility was good. Oxford-cup method has high quality and good reproducibility. The K-B rule is only suitable for qualitative test. When the bacteriostasis test was carried out, it was suggested that the test plate should be prepared by the method of pre-inoculation of bacterial liquid, the concentration of bacteria in the test plate should be 1 脳 10 ~ (5) 渭 m ~ (-1) 脳 10 ~ (-6) CFU/m / L, and the appropriate concentration should be selected for the test drug. After adding the experimental drugs, the culture should be carried out in the incubator for about 2 hours before diffusion at 4 鈩，

本文编号：2214762