不同贮存时间的单采少白细胞血小板中精氨酸酶水平的研究
发布时间：2018-09-07 15:44
【摘要】：目的:探讨不同贮存时间对单采少白细胞血小板(apheresis leukocyte-reduced platelet,ALR-Plt)中精氨酸酶水平的影响及精氨酸酶的可能来源。方法:应用ELISA法检测不同贮存时间ALR-Plt和对照血浆中精氨酸酶水平及髓过氧化物酶(myeloperoxidase,MPO)水平;应用相关性分析分析不同贮存时间的ALR-Plt中精氨酸酶水平与MPO水平的相关性。结果:贮存3 d内的ALR-Plt与对照血浆中精氨酸酶水平差异无统计学意义,但是贮存超过3d的ALR-Plt中精氨酸酶水平显著高于贮存3 d内的ALR-Plt和对照血浆中的水平(P0.05)。不同贮存时间的ALR-Plt之间MPO水平差异无统计学意义,但均显著低于对照血浆中的水平(P0.05)。相关性分析显示:ALRPlt中精氨酸酶水平与MPO水平呈正相关(r=0.58)。结论:贮存时间超过3 d的ALR-Plt中精氨酸酶水平显著升高,其可能来源于残留的少量白细胞,特别是中性粒细胞。
[Abstract]:Aim: to investigate the effect of different storage time on argininase level in single white blood cell platelets (apheresis leukocyte-reduced platelet,ALR-Plt) and the possible sources of argininase. Methods: the levels of arginase and myeloperoxidase (myeloperoxidase,MPO) in ALR-Plt and control plasma were detected by ELISA method, and the correlation between arginase level and MPO level in ALR-Plt with different storage time was analyzed. Results: there was no significant difference in arginase level between ALR-Plt and control plasma after 3 days of storage, but the arginase level in ALR-Plt stored for more than 3 days was significantly higher than that in ALR-Plt and control plasma (P0.05). There was no significant difference in MPO levels between ALR-Plt at different storage time, but they were significantly lower than those in control plasma (P0.05). Correlation analysis showed that argininase level was positively correlated with MPO level (r = 0. 58). Conclusion: the level of argininase in ALR-Plt for more than 3 days may be derived from a small amount of residual white blood cells, especially neutrophils.
【作者单位】： 福建医科大学附属协和医院输血科;福建医科大学附属协和医院血液科、福建省血液病研究所;
【基金】：福建省中青年教师教育科研项目(JA14133) 2015年福建省卫生系统中青年骨干人才培养项目基础项目(2015-ZQN-JC-18) 2016年福建省自然科学基金计划项目(2016J01461)
【分类号】：R457.1
，
本文编号：2228693
[Abstract]:Aim: to investigate the effect of different storage time on argininase level in single white blood cell platelets (apheresis leukocyte-reduced platelet,ALR-Plt) and the possible sources of argininase. Methods: the levels of arginase and myeloperoxidase (myeloperoxidase,MPO) in ALR-Plt and control plasma were detected by ELISA method, and the correlation between arginase level and MPO level in ALR-Plt with different storage time was analyzed. Results: there was no significant difference in arginase level between ALR-Plt and control plasma after 3 days of storage, but the arginase level in ALR-Plt stored for more than 3 days was significantly higher than that in ALR-Plt and control plasma (P0.05). There was no significant difference in MPO levels between ALR-Plt at different storage time, but they were significantly lower than those in control plasma (P0.05). Correlation analysis showed that argininase level was positively correlated with MPO level (r = 0. 58). Conclusion: the level of argininase in ALR-Plt for more than 3 days may be derived from a small amount of residual white blood cells, especially neutrophils.
【作者单位】： 福建医科大学附属协和医院输血科;福建医科大学附属协和医院血液科、福建省血液病研究所;
【基金】：福建省中青年教师教育科研项目(JA14133) 2015年福建省卫生系统中青年骨干人才培养项目基础项目(2015-ZQN-JC-18) 2016年福建省自然科学基金计划项目(2016J01461)
【分类号】：R457.1
，
本文编号：2228693
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