基于金磁微粒的TORCH-IgM四项免疫层析检测方法的建立
发布时间：2018-09-07 22:23
【摘要】：TORCH是围产期感染的一组疾病的病原体缩写,包括弓形虫、风疹病毒、巨细胞病毒和单纯疱疹病毒。它们所引起的先天感染会对胎儿造成严重伤害。孕妇感染TORCH病原体后一般没有明显临床症状,即使有症状,也缺乏特异性。另一方面,特异性IgM抗体作为免疫应答中首先出现的抗体,是病原体早期检测的一个标志。因而检测TORCH特异性IgM抗体可实现对TORCH病原体的早期检测,具有较高的临床价值。本文以GoldMag(?)金磁微粒为载体,将其分别进行聚苯乙烯磺酸钠(PSS)修饰和聚甲基丙烯酸钠(PMAA)修饰。修饰后的GoldMag(?)金磁微粒可与相应的鼠抗人IgM(μ链特异)抗体相结合形成探针,进而建立了一种基于GoldMag(?)金磁微粒的TORCH-IgM四项免疫层析检测方法,以实现对TORCH病原体的快速检测。结果表明,PMAA修饰后的GoldMag(?)金磁微粒粒径明显变小且分布均一。将修饰后的GoldMag(?)金磁微粒与鼠抗人IgM抗体结合物用于免疫层析检测TORCH-IgM发现,此方法具有重复性好、灵敏度高、特异性强的特点。甘油三酯、血红素、胆红素等常见干扰物质对检测结果无影响且四种病原体之间没有交叉反应。与市售TORCH-IgM检测试剂盒相比,此方法具有更高的灵敏度。
[Abstract]:TORCH is an acronym for a group of perinatal infections, including Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex virus. The congenital infections they cause can cause serious damage to the fetus. Pregnant women infected with TORCH pathogens generally have no obvious clinical symptoms, even if there are symptoms, also lack specificity. On the other hand, specific IgM antibody, as the first antibody in immune response, is a marker for early detection of pathogens. Therefore, the detection of TORCH specific IgM antibody can realize the early detection of TORCH pathogens, which has high clinical value. In this paper, GoldMag (?) Gold magnetic particles were modified by sodium polystyrene sulfonate (PSS) and poly (sodium methacrylate) (PMAA) respectively. Modified GoldMag (?) The gold magnetic particle can be combined with the corresponding mouse anti-human IgM (渭 chain specific) antibody to form a probe, and a novel GoldMag (?) Four TORCH-IgM immunochromatographic methods were used to detect gold magnetic particles in order to detect TORCH pathogens quickly. The results showed that GoldMag (?) The particle size of gold magnetic particle is obviously smaller and the distribution is uniform. The modified GoldMag (?) The combination of gold magnetic particle and mouse anti-human IgM antibody was used to detect TORCH-IgM by immunochromatography. The method has the advantages of good reproducibility, high sensitivity and strong specificity. Triglycerides, heme, bilirubin and other common interfering substances have no effect on the detection results and there is no cross reaction among the four pathogens. Compared with the commercial TORCH-IgM detection kit, this method has higher sensitivity.
【学位授予单位】：西北大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.6
本文编号：2229581
[Abstract]:TORCH is an acronym for a group of perinatal infections, including Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex virus. The congenital infections they cause can cause serious damage to the fetus. Pregnant women infected with TORCH pathogens generally have no obvious clinical symptoms, even if there are symptoms, also lack specificity. On the other hand, specific IgM antibody, as the first antibody in immune response, is a marker for early detection of pathogens. Therefore, the detection of TORCH specific IgM antibody can realize the early detection of TORCH pathogens, which has high clinical value. In this paper, GoldMag (?) Gold magnetic particles were modified by sodium polystyrene sulfonate (PSS) and poly (sodium methacrylate) (PMAA) respectively. Modified GoldMag (?) The gold magnetic particle can be combined with the corresponding mouse anti-human IgM (渭 chain specific) antibody to form a probe, and a novel GoldMag (?) Four TORCH-IgM immunochromatographic methods were used to detect gold magnetic particles in order to detect TORCH pathogens quickly. The results showed that GoldMag (?) The particle size of gold magnetic particle is obviously smaller and the distribution is uniform. The modified GoldMag (?) The combination of gold magnetic particle and mouse anti-human IgM antibody was used to detect TORCH-IgM by immunochromatography. The method has the advantages of good reproducibility, high sensitivity and strong specificity. Triglycerides, heme, bilirubin and other common interfering substances have no effect on the detection results and there is no cross reaction among the four pathogens. Compared with the commercial TORCH-IgM detection kit, this method has higher sensitivity.
【学位授予单位】：西北大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.6
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