微量板法与试管法检测ABO血型抗体效价的比较

发布时间：2018-09-18 16:58

【摘要】：目的比较微量板法与试管法检测ABO血型Ig M抗体效价的差异。方法使用微柱凝集法对129例健康供者和91例造血干细胞移植患者进行ABO血型正反定型鉴定,根据反定型结果,同1标本同时经试管法与微量板法进行ABO血型Ig M抗体效价测定并进行比较。结果 220例被研究者中,162例标本需要进行抗体效价检测。抗-A/抗-B效价在不同病例群体中,2种方法检测的一致性差异不具有统计学意义(P0.05);2种方法检测造血干细胞移植患者ABO血型抗体效价、健康供者抗-A效价的差异不具有统计学意义(P0.05);微量板法测得的健康供者抗-B效价明显低于试管法(17.78±3.02 vs 19.05±2.95,P0.05)。结论全自动微量板法微量板法可广泛用于造血干细胞移植患者ABO血型Ig M抗体效价的检测,而用于健康供者抗-B效价检测时抗体效价低于试管法,必要时需要增加试管法。
[Abstract]:Objective to compare the titer of ABO blood group Ig M antibody between microplate method and test tube method. Methods using microcolumn agglutination method, 129 healthy donors and 91 patients with hematopoietic stem cell transplantation (HSCT) were identified by positive and negative typing of ABO blood group. The titers of Ig M antibody of ABO blood group were determined and compared by the method of test tube and microplate at the same time. Results 162 samples of 220 subjects need to be tested for antibody titer. There was no significant difference in the consistency between the two methods in detecting the titer of ABO blood group in patients with hematopoietic stem cell transplantation (P0.05), and there was no significant difference between the two methods in detecting the titer of ABO blood group antibody in patients with hematopoietic stem cell transplantation (HSCT). There was no significant difference in anti--A titer of healthy donors (P0.05), and the anti-B titer of healthy donors measured by microplate method was significantly lower than that of test tube method (17.78 卤3.02 vs 19.05 卤2.95 P 0.05). Conclusion automatic microplate method can be widely used in the detection of ABO blood group Ig M antibody titer in patients with hematopoietic stem cell transplantation, but the antibody titer of anti-B antibody in healthy donors is lower than that of the test tube method, and it is necessary to increase the test tube method if necessary.
【作者单位】：北京大学人民医院输血科;
【基金】：北京大学人民医院研究与发展基金(2118000595)
【分类号】：R457.1

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