大蒜素与不同抗菌药物联用对耐甲氧西林金黄色葡萄球菌的体外抗菌活性研究
发布时间：2018-11-01 16:50
【摘要】：目的研究大蒜素与抗菌药物联用对耐甲氧西林金黄色葡萄球菌(MRSA)的体外抗菌活性,为临床治疗提供参考依据。方法采用微量肉汤稀释法分别测定大蒜素与抗菌药物对30株MRSA的最低抑菌浓度(MIC),考察单用两药对MRSA的抗菌活性;以测得的单药MIC为依据,采用棋盘法设计,测定并计算大蒜素和抗菌药物联用对30株MRSA的部分抑菌浓度指数(FIC),考察两药联合对MRSA的抗菌活性。结果单用大蒜素对30株MRSA的MIC为(0.30±0.09)mg/mL,与苯唑西林、头孢唑林联用后使其MIC50由64μg/mL和32μg/mL下降到8μg/mL,以协同或相加作用为主;与万古霉素联用后使其MIC50由2μg/mL下降到0.5μg/mL,主要表现为相加作用。结论大蒜素对MRSA有一定抑制作用,与抗菌药物联合后能增强其抑菌效果,临床上可考虑使用大蒜素作为抗菌药物,特别是万古霉素的增敏剂用于治疗MRSA引起的严重感染。
[Abstract]:Objective to study the in vitro antibacterial activity of allicin combined with antimicrobial agents against methicillin-resistant Staphylococcus aureus (MRSA). Methods the minimal inhibitory concentration (MIC),) of allicin and antimicrobial agents on 30 MRSA strains was determined by broth dilution method. The antibacterial activity of allicin and antimicrobial agents against MRSA was investigated by (MIC),. Based on the single drug MIC, the antimicrobial activity of allicin and antimicrobial agents on 30 MRSA strains was determined and calculated by chessboard design. (FIC), was used to investigate the antibacterial activity of the two drugs against MRSA. Results the MIC of 30 MRSA strains treated with allicin alone was (0.30 卤0.09) mg/mL, and oxacillin, and the MIC50 of cefazolin decreased from 64 渭 g/mL and 32 渭 g/mL to 8 渭 g / mL, which was mainly synergistic or additive. In combination with vancomycin, the MIC50 decreased from 2 渭 g/mL to 0. 5 渭 g / mL, which mainly showed additive effect. Conclusion allicin has a certain inhibitory effect on MRSA, combined with antimicrobial agents can enhance its bacteriostatic effect. In clinic, allicin can be considered as an antimicrobial agent, especially vancomycin sensitizer for the treatment of severe infection caused by MRSA.
【作者单位】： 嘉兴市妇幼保健院药剂科;嘉兴市妇幼保健院检验科;
【基金】：嘉兴市科技计划资助项目(2014AY21047)
【分类号】：R446.5
本文编号：2304514
[Abstract]:Objective to study the in vitro antibacterial activity of allicin combined with antimicrobial agents against methicillin-resistant Staphylococcus aureus (MRSA). Methods the minimal inhibitory concentration (MIC),) of allicin and antimicrobial agents on 30 MRSA strains was determined by broth dilution method. The antibacterial activity of allicin and antimicrobial agents against MRSA was investigated by (MIC),. Based on the single drug MIC, the antimicrobial activity of allicin and antimicrobial agents on 30 MRSA strains was determined and calculated by chessboard design. (FIC), was used to investigate the antibacterial activity of the two drugs against MRSA. Results the MIC of 30 MRSA strains treated with allicin alone was (0.30 卤0.09) mg/mL, and oxacillin, and the MIC50 of cefazolin decreased from 64 渭 g/mL and 32 渭 g/mL to 8 渭 g / mL, which was mainly synergistic or additive. In combination with vancomycin, the MIC50 decreased from 2 渭 g/mL to 0. 5 渭 g / mL, which mainly showed additive effect. Conclusion allicin has a certain inhibitory effect on MRSA, combined with antimicrobial agents can enhance its bacteriostatic effect. In clinic, allicin can be considered as an antimicrobial agent, especially vancomycin sensitizer for the treatment of severe infection caused by MRSA.
【作者单位】： 嘉兴市妇幼保健院药剂科;嘉兴市妇幼保健院检验科;
【基金】：嘉兴市科技计划资助项目(2014AY21047)
【分类号】：R446.5
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1 朱春梅;曹茂堂;侯均;;抗菌药物联用及细菌培养送检情况分析[J];中国医院药学杂志;2006年12期
，本文编号：2304514
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