B群链球菌多位点序列型及大环内酯类耐药基因相关性分析

发布时间：2018-11-02 15:52

【摘要】：目的对孕妇携带的大环内酯类耐药的B群链球菌(GBS)进行多位点序列分型及大环内酯类耐药基因检测,探讨其多位点序列型与大环内酯类耐药基因的相关性。方法采用PCR方法检测东莞地区孕妇携带的82株大环内酯类耐药GBS的耐药基因erm B、mef A/E、lin B。采用PCR扩增82株大环内酯类耐药GBS的7个管家基因,对其进行DNA测序,并对测序结果进行多位点序列分型(MLST),以e BURSTv3.0分析菌株间的亲缘关系。结果 82株大环内酯类耐药的GBS中,耐药基因erm B、mef A/E和lin B的总检出率分别为73.17%、46.34%和7.32%。MLST分型得到CC1、CC12、CC17、CC19共4个克隆群;17个序列型,主要序列型为ST1(6.10%)、ST12(7.32%)、ST17(9.76%)、ST19(37.8%)和ST23(14.63%)。结论东莞地区孕妇携带的序列型ST19的GBS检测率高;序列型ST12、ST19、ST23的GBS大环内酯类耐药基因携带率高,其分子构型可能与大环内酯类耐药基因的获得关系密切。
[Abstract]:Objective to study the multilocus sequence typing and the detection of macrolide resistance genes of streptococcus B carrying macrolide resistance in pregnant women, and to explore the correlation between the multilocus sequence type and macrolide resistant genes. Methods 82 strains of macrolide resistant GBS were detected by PCR method. The 7 housekeeping genes of 82 macrolide resistant GBS strains were amplified by PCR and sequenced by DNA. The results were sequenced by multilocus sequence typing (MLST), to analyze the relationship between the strains. Results in 82 GBS strains with macrolide resistance, the total detection rates of erm Bnmef A / E and lin B were 73.176.34% and 46.34%, respectively, and 4 clones of CC1,CC12,CC17,CC19 were obtained by 7.32%.MLST typing. The main sequence types were ST1 (6.10%), ST12 (7.32%), ST17 (9.76%), ST19 (37.8%) and ST23 (14.63%). Conclusion the detection rate of GBS of the sequence ST19 carried by pregnant women in Dongguan area is high, and the carrying rate of GBS macrolide resistance gene of sequence type ST12,ST19,ST23 is high, and its molecular configuration may be closely related to the acquisition of macrolide resistance gene.
【作者单位】：广东医科大学医学检验研究所临床微生物学教研室;中山大学附属东华医院检验科细菌室;
【基金】：东莞市社会科技发展项目(No.2014108101002)
【分类号】：R440

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