肺炎克雷伯菌生物膜形成能力及其与多黏菌素B耐药相关性研究

发布时间：2018-11-04 08:53

【摘要】：目的探究肺炎克雷伯菌生物膜形成能力及其与多黏菌素B耐药相关性。方法收集温州医科大学附属第一医院临床分离的耐碳青霉烯类肺炎克雷伯菌(carbapenem-resistant Klebsiella pneumonia,CRKPs)共37株,琼脂稀释法检测亚胺培南(imipenem,IMP)、美罗培南(meropenem,MEP)、厄他培南(ertapenen,ETP)和多黏菌素B(polymyxin B,PB)对37株试验菌株的最低抑菌浓度(minimum inhibition concentrations,MICs);96孔板结晶紫染色法检测37株试验菌株的生物膜A590值;检测37株试验菌株对PB的生物膜最低消除浓度(minimal biofi lm elimination concentrations,MBECs)。结果 37株CRKPs对3种碳青霉烯类药物的敏感性都表现出不同程度的下降,对PB保持敏感,MIC为0.25~1μg/m L,而MBEC为3~12μg/m L,相对于MIC值都表现为耐药;但是生物膜形成能力的强弱与MBEC值之间不存在线性相关性。结论本次37株耐碳青霉烯肺炎克雷伯菌形成生物膜后可以增加对PB的耐药,但是生物膜形成能力的强弱与耐药程度无关。
[Abstract]:Objective to investigate the biofilm forming ability of Klebsiella pneumoniae and its correlation with polymyxin B resistance. Methods A total of 37 clinical isolates of Klebsiella pneumoniae resistant to carbapenem (carbapenem-resistant Klebsiella pneumonia,CRKPs) were collected from the first affiliated Hospital of Wenzhou Medical University. Imipenem (imipenem,IMP) and meropenem (meropenem,MEP) were detected by Agar dilution assay. The minimum inhibitory concentration (minimum inhibition concentrations,MICs) of ertapenen,ETP and B (polymyxin against 37 tested strains; The biofilm A590 values of 37 strains were detected by 96 hole plate crystal violet staining method, and the minimum biofilm elimination concentration (minimal biofi lm elimination concentrations,MBECs) of 37 tested strains to PB was detected. Results the sensitivity of 37 CRKPs strains to three carbapenes decreased in varying degrees and remained sensitive to PB. The MIC was 0.25 渭 g / mL, while the MBEC was 312 渭 g / mL, which was resistant to MIC. However, there is no linear correlation between biofilm formation ability and MBEC value. Conclusion the biofilm resistance to PB was increased in 37 strains of Klebsiella pneumoniae resistant to carbapenem, but the biofilm forming ability was not related to the degree of drug resistance.
【作者单位】：温州医科大学检验医学院与生命科学学院;浙江医院检验科;温州医科大学附属第一医院医学检验中心;
【基金】：国家自然科学基金(No.81171614) 浙江省卫生高层次创新人才培养工程项目(浙卫发No.[2012]241号)
【分类号】：R446.5

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