动物源肺炎克雷伯菌耐药性及MLST分析

发布时间：2018-11-12 18:13

【摘要】：为了解本地区动物源肺炎克雷伯菌耐药性及分子流行病学特征,本研究收集分离于4种动物的肺炎克雷伯菌67株,采用K-B纸片扩散法和肉汤稀释法测定其对15种常用抗生素的敏感性,并采用多位点序列分型技术(MLST)对所有菌株进行分子遗传学分析;同时利用e BURST软件对多位点序列分型结果进行分析。药敏结果显示,67株动物源肺炎克雷伯菌对15株常用抗生素的耐药率为2.99%~61.19%,多重耐药菌株占40.3%,不同来源的菌株耐药性存在明显差异。MLST分析表明,全部菌株共分成17个ST型,其中鸡源菌株有5个(ST235、ST37、ST2019、ST2021、ST726)、猪源菌株有6个(ST258、ST11、ST270、ST106、ST1863、ST263)、兔源菌株有4个(ST17、ST148、ST60、ST168)、犬源菌株有3个(ST11、ST65、ST74),其中ST11为猪源菌株和犬源菌株共有的类型。结果表明肺炎克雷伯菌在不同动物中的流行表现一定的分子差异,对常用抗生素的耐药呈现多重耐药的趋势。本研究将肺炎克雷伯菌的耐药表型与MLST分子型结合进行调查研究,揭示二者间关系,为进一步开展肺炎克雷伯菌分子流行病学研究、防控耐药菌株的暴发流行提供依据。
[Abstract]:In order to understand the drug resistance and molecular epidemiological characteristics of Klebsiella pneumoniae isolated from four animals, 67 strains of Klebsiella pneumoniae were collected in this study. K-B disk diffusion method and broth dilution method were used to determine the sensitivity to 15 common antibiotics, and (MLST) was used to analyze the molecular genetics of all strains. At the same time, e BURST software was used to analyze the results of multi-locus sequence typing. The results showed that the resistance rate of 67 strains of Klebsiella pneumoniae to 15 commonly used antibiotics was 2.99%, and that of multidrug resistant strains was 40.33%. There were significant differences in drug resistance of different strains from different sources. MLST analysis showed that there were significant differences in drug resistance between different strains of Klebsiella pneumoniae. All the strains were divided into 17 ST types, including 5 strains from chicken (ST235,ST37,ST2019,ST2021,ST726), 6 strains from pigs (ST258,ST11,ST270,ST106,ST1863,ST263), 4 strains from rabbits (ST17,ST148,ST60,ST168), 5 strains from chicken (ST235,ST37,ST2019,ST2021,ST726), 6 strains from pigs (ST258,ST11,ST270,ST106,ST1863,ST263) and 4 strains from rabbits (ST17,ST148,ST60,ST168). There are three canine strains (ST11,ST65,ST74), among which ST11 is a common type of porcine and canine strains. The results showed that the prevalence of Klebsiella pneumoniae in different animals showed certain molecular differences, and the resistance to common antibiotics showed a trend of multidrug resistance. In this study, the drug resistant phenotype of Klebsiella pneumoniae and the molecular type of MLST were investigated and studied to reveal the relationship between them, and to provide the basis for further research on molecular epidemiology of Klebsiella pneumoniae and the prevention and control of the outbreak of resistant strains.
【作者单位】：新乡医学院微生物学教研室;新乡医学院第三附属医院检验科;
【基金】：河南省高等学校青年骨干教师资助项目(2015GGJS-134)
【分类号】：R446.5

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