HIV血清学假阳性献血者的归队条件探究
发布时间：2018-11-15 15:51
【摘要】：目的排除HIV血清学筛查中的假阳性献血者,探讨HIV血清学反应性献血者的归队条件。方法对2013年1月1日-2015年6月30日间大连市血液中心常规血液筛查中核酸检测(NAT)无反应性、HIV血清学反应性且不被免疫印迹法(WB)确证的献血者做归队复查。复查方式同血液筛查,但血清学检测不设"灰区"。结果共筛查出HIV血清学反应性且确证阳性83例,流行率为0.43‰(83/192 065),其中第3代ELISA漏检4例(漏检率0.2/万),第4代ELISA漏检2例(漏检率0.1/万);ELISA试剂设置"灰区"的特异性低于取消"灰区"的特异性(特异性差D的95%可信区间:第3代试剂0.06‰-0.17‰,第4代试剂0.18‰-0.32‰);研究期间归队复查献血者68人,归队检测总次数为112次,单次归队复查的时间跨度为47-867(中位数194)d,单人归队的时间总跨度为89-908(中位数373)d。41人仅做了1次复查,合格率为70.73%(29/41);14人做了2次复查,合格率为57.14%(8/14);10人做了3次复查,90.00%(9/10);3人做了≥4次复查,合格率为33.33%(1/3)。归队反应性标本的确证试验全部为阴性。结论采用NAT和第4代ELISA联合检测模式同时取消HIV血清学检测的"灰区"可以减少血液筛查及归队检测中的假阳性,利于更多的献血者归队;血清学HIV检测反应性献血者的归队标准宜为每次复查间隔时间≥3个月,2次复查合格者即可再次献血。
[Abstract]:Objective to exclude false positive blood donors from HIV serological screening and to explore the conditions of return of HIV serological reactive donors. Methods from January 1, 2013 to June 30, 2015, donors whose nucleic acids were tested for (NAT) nonreactivity and HIV serological reactivity and were not confirmed by Western blot (WB) were reviewed. The method of reexamination was the same as blood screening, but no gray area was set up for serological examination. Results 83 cases of HIV seroreactivity were screened and confirmed positive, the prevalence rate was 0.43 鈥，
本文编号：2333736
[Abstract]:Objective to exclude false positive blood donors from HIV serological screening and to explore the conditions of return of HIV serological reactive donors. Methods from January 1, 2013 to June 30, 2015, donors whose nucleic acids were tested for (NAT) nonreactivity and HIV serological reactivity and were not confirmed by Western blot (WB) were reviewed. The method of reexamination was the same as blood screening, but no gray area was set up for serological examination. Results 83 cases of HIV seroreactivity were screened and confirmed positive, the prevalence rate was 0.43 鈥，
本文编号：2333736
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