肺炎链球菌毒力基因检测与临床研究

发布时间：2018-12-17 14:25

【摘要】：目的了解肺炎链球菌10种常见毒力基因(pspA、pspC、lytA、lytB、rrgA、nanA、pav、iga、ply、hysA)在临床分离菌株中的分布,为研究其在致病过程中的作用机制、新型疫苗研制提供参考。方法选取2013年1月-2014年12月从南京医科大学附属无锡人民医院临床分离的20株肺炎链球菌为研究菌株,采用PCR法对10种靶基因进行检测,统计并分析各毒力基因表达阳性率及其与细菌耐药性之间的关系,数据采用SPSS 17.0软件进行统计分析。结果 10种毒力基因在20株肺炎链球菌中表达阳性率分别为0、0、45.0%、60.0%、35.0%、60.0%、75.0%、80.0%、55.0%及55.0%,其中pav、iga阳性率对较高;红霉素耐药菌株中rrgA及pav的阳性率分别为64.5%、100.0%,显著高于非耐药菌株(P0.05)。结论 10种毒力基因在肺炎链球菌中有不同程度的表达,其中pav、iga阳性率较高,可能成为新型疫苗的候选分子;rrgA及pav可能与肺炎链球菌对红霉素耐药有关。
[Abstract]:Objective to investigate the distribution of 10 common virulence genes (pspA,pspC,lytA,lytB,rrgA,nanA,pav,iga,ply,hysA) of Streptococcus pneumoniae in clinical isolates and to provide a reference for the study of the mechanism of its role in the pathogenic process and the development of a new vaccine. Methods from January 2013 to December 2014, 20 strains of Streptococcus pneumoniae isolated from Wuxi people's Hospital of Nanjing Medical University were selected as study strains, and 10 target genes were detected by PCR assay. The positive rate of each virulence gene and its relationship with bacterial resistance were statistically analyzed by SPSS 17.0 software. Results the positive rates of 10 virulence genes in 20 strains of Streptococcus pneumoniae were 0 0 / 45.0 and 60.0 / 60.050.0 / 60.055.0% and 55.0%, respectively, and pav, was found in 55.0% and 55.0% of the 20 strains of Streptococcus pneumoniae. The positive rate of iga was higher. The positive rates of rrgA and pav in erythromycin resistant strains were 64.5% and 100.0 respectively, which were significantly higher than those of non-resistant strains (P0.05). Conclusion Ten virulence genes are expressed in different degree in Streptococcus pneumoniae, among them, the positive rate of pav,iga is higher, and rrgA and pav may be the candidate molecules of new vaccine, and rrgA and pav may be related to the resistance of Streptococcus pneumoniae to erythromycin.
【作者单位】：南京医科大学附属无锡人民医院呼吸内科;南京医科大学附属无锡人民医院临床检验科;无锡市克隆遗传技术研究所;
【基金】：江苏省科技厅基金资助项目(BL2014023)
【分类号】：R446.5

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