TNF-α抑制剂对神经病理性痛大鼠镇痛作用及其机制探讨
发布时间：2019-01-14 07:19
【摘要】：目的:探究肿瘤坏死因子α(tumor necrosis factorα,TNF-α)抑制剂XPro~汶1595对大鼠脊神经结扎诱导的痛行为及脊髓背角白介素1β(IL-1β)、白介素6(IL-6)和TNF-α表达的影响。方法:运用L5脊神经结扎的方法建立神经病理性痛模型,通过鞘内注射给予TNF-α抑制剂(XPro~汶1595)进行干预,采用Von Frey丝测定大鼠足底50%机械缩足反射阈值;运用Western Blot方法检测大鼠脊髓背角IL-1β、IL-6和TNF-α的表达情况。结果:(1)行为学结果显示:与对照组相比,脊神经结扎后大鼠表现出明显的机械性痛敏(P0.01),且可被鞘内注射XPro~汶1595有效改善(P0.01);(2)Western Blot结果显示:与对照组相比,脊神经结扎组大鼠脊髓背角IL-1β、IL-6和TNF-α的表达显著升高,且可被鞘内注射XPro~汶1595明显抑制(P0.01)。结论:鞘内注射XPro~汶1595能够显著改善大鼠脊神经结扎诱导的神经病理性痛,其机制与抑制脊髓背角IL-1β、IL-6和TNF-α等炎性细胞因子的表达有关。
[Abstract]:Aim: to investigate the effects of tumor necrosis factor 伪 (TNF- 伪) inhibitor XPro~ Wen 1595 on the pain induced by spinal nerve ligation and the expression of interleukin 1 尾 (IL-1 尾), interleukin 6 (IL-6) and TNF- 伪 in the spinal dorsal horn of rats. Methods: the neuropathic pain model was established by ligation of L5 spinal nerve. TNF- 伪 inhibitor (XPro~ 1595) was injected intrathecally, and the reflex threshold of 50% mechanical foot contraction was measured by Von Frey wire. The expression of IL-1 尾, IL-6 and TNF- 伪 in spinal dorsal horn of rats was detected by Western Blot method. Results: (1) Behavioral results showed that compared with the control group, the spinal nerve ligation rats showed obvious mechanical pain sensitivity (P0.01), and the intrathecal injection of XPro~ Wen 1595 could be effectively improved (P0.01). (2) Western Blot results showed that the expression of IL-1 尾, IL-6 and TNF- 伪 in spinal dorsal horn of spinal cord ligation group was significantly higher than that in control group, and could be inhibited by intrathecal injection of XPro~ Wen 1595 (P0.01). Conclusion: intrathecal injection of XPro~ Wen 1595 can significantly improve the neuropathic pain induced by spinal nerve ligation in rats, and its mechanism is related to the inhibition of the expression of inflammatory cytokines such as IL-1 尾, IL-6 and TNF- 伪 in the dorsal horn of spinal cord.
【作者单位】： 第四军医大学人体解剖学教研室暨梁挾琚脑研究中心;第四军医大学学员一旅;第四军医大学西京医院麻醉科;
【基金】：国家自然科学基金(81171050,81171051)
【分类号】：R402
本文编号：2408439
[Abstract]:Aim: to investigate the effects of tumor necrosis factor 伪 (TNF- 伪) inhibitor XPro~ Wen 1595 on the pain induced by spinal nerve ligation and the expression of interleukin 1 尾 (IL-1 尾), interleukin 6 (IL-6) and TNF- 伪 in the spinal dorsal horn of rats. Methods: the neuropathic pain model was established by ligation of L5 spinal nerve. TNF- 伪 inhibitor (XPro~ 1595) was injected intrathecally, and the reflex threshold of 50% mechanical foot contraction was measured by Von Frey wire. The expression of IL-1 尾, IL-6 and TNF- 伪 in spinal dorsal horn of rats was detected by Western Blot method. Results: (1) Behavioral results showed that compared with the control group, the spinal nerve ligation rats showed obvious mechanical pain sensitivity (P0.01), and the intrathecal injection of XPro~ Wen 1595 could be effectively improved (P0.01). (2) Western Blot results showed that the expression of IL-1 尾, IL-6 and TNF- 伪 in spinal dorsal horn of spinal cord ligation group was significantly higher than that in control group, and could be inhibited by intrathecal injection of XPro~ Wen 1595 (P0.01). Conclusion: intrathecal injection of XPro~ Wen 1595 can significantly improve the neuropathic pain induced by spinal nerve ligation in rats, and its mechanism is related to the inhibition of the expression of inflammatory cytokines such as IL-1 尾, IL-6 and TNF- 伪 in the dorsal horn of spinal cord.
【作者单位】： 第四军医大学人体解剖学教研室暨梁挾琚脑研究中心;第四军医大学学员一旅;第四军医大学西京医院麻醉科;
【基金】：国家自然科学基金(81171050,81171051)
【分类号】：R402
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