梅毒孕妇血清与乳汁及唾液梅毒螺旋体DNA的检测分析

发布时间：2019-01-15 21:49

【摘要】：目的探讨梅毒孕妇经规范治疗后血清、乳汁、唾液标本中梅毒螺旋体DNA(TP-DNA)含量,评估母亲体液的传染性,为此类患者产后能否哺乳提供更加充分科学依据。方法选取2015年1月-10月规范治疗两个疗程梅毒孕妇108例,根据治疗是否有效分为治疗有效组50例,无明显变化组58例,用荧光定量PCR检测其血清、乳汁、唾液TP-DNA,同时进行梅毒螺旋体抗体快速血浆反应素试验(RPR)。结果梅毒孕妇规范治疗两个疗程后,血清、乳汁、唾液TP-DNA阳性率及血清RPR阳性率分别为13.9%、13.0%、12.0%、53.7%;梅毒孕妇经规范治疗后,RPR无明显变化组孕妇血清、乳汁、唾液TP-DNA阳性率分别为22.41%、20.70%、18.97%,分别高于治疗有效组,差异均有统计学意义(P0.05);两组组内血清、乳汁和唾液中的TP-DNA阳性率之间比较差异无统计学意义;按照血清RPR滴度分组,随着孕妇血清中RPR滴度升高,其血清、乳汁、唾液TP-DNA阳性检出率越来越高,各组血清、乳汁及唾液TP-DNA阳性率差异均有统计学意义(P0.05)。结论梅毒孕妇经规范治疗后是否具有传染性与血清、乳汁、唾液TP-DNA载量有关;治疗有效组仍有部分乳汁、唾液中检出TPDNA阳性,乳汁、唾液的TP-DNA检测从一定程度上可代替血TP-DNA,优点为取材方便,产妇易接受,更有安全感。
[Abstract]:Objective to investigate the content of DNA (TP-DNA) in serum, milk and saliva of pregnant women with syphilis after regular treatment, and to evaluate the infectivity of mother's body fluid, so as to provide more scientific basis for postpartum lactation. Methods 108 pregnant women with syphilis from January to October 2015 were divided into two groups according to whether the treatment was effective or not. The serum, milk and saliva TP-DNA, of 108 pregnant women were detected by fluorescence quantitative PCR. Simultaneous rapid plasma reactin test for Treponema pallidum antibody (RPR). Results after two courses of standardized treatment for pregnant women with syphilis, the positive rates of serum, milk, saliva TP-DNA and serum RPR were 13.90.13.0 and 12.0and 53.7, respectively. The positive rates of TP-DNA in serum, milk and saliva of pregnant women with syphilis after standardized treatment were 22.41%, 20.70% and 18.97, respectively, which were significantly higher than those of effective group (P0.05). There was no significant difference in TP-DNA positive rate between the two groups in serum, milk and saliva. According to the titer of serum RPR, with the increase of serum RPR titer of pregnant women, the positive rate of TP-DNA in serum, milk and saliva was higher and higher. The positive rates of TP-DNA in serum, milk and saliva were significantly different among the groups (P0.05). Conclusion the TP-DNA load of serum, milk and saliva is related to the infectivity of pregnant women with syphilis after standardized treatment. In the effective group, there were still some milk, TPDNA positive in saliva, TP-DNA detection of milk, saliva can replace the advantages of blood TP-DNA, to a certain extent, easy to obtain material, maternal easy to accept, more sense of security.
【作者单位】：宁波市妇女儿童医院检验科;宁波市妇女儿童医院皮肤科;宁波市妇女儿童医院妇产科;
【基金】：浙江省医药卫生科技计划基金资助项目(2014KYB357) 宁波市科技局.宁波市母胎医学研究重点实验室基金资助项目(2010A22011)
【分类号】：R714.25;R440

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