病原褪色沙雷氏菌主要生物学性状研究
发布时间：2019-02-27 17:48
【摘要】：对从河北某些经济昆虫养殖场发病(死亡)蝗虫(东亚飞蝗)中分离的褪色沙雷氏菌(Serratia marcescens),进行了比较系统的主要生物学性状的研究,主要包括形态学特征、理化特性、系统发育学、病原学意义、血清学分型、脉冲场凝胶电泳分子分型等方面的内容。研究结果显示,来源于发病(死亡)养殖蝗虫的褪色沙雷氏菌,各菌株具有相同的基本表观生物学指征性状和系统发育特征,但存在产生、不产生红色色素的菌株。通过对健康蝗虫的回归感染实验,按 科赫法则‖进行病原学意义判定,表明分离菌株具有比较强的致病作用,也是构成蝗虫自然发病(死亡)的原发病原菌。选择从不同蝗虫养殖场分离获得的病原褪色沙雷氏菌代表菌株,采用经典的免疫血清学凝集试验方法进行血清学同源性分析,表明在不同菌株间存在同源性差异性,但均存在显著的血清学交叉反应。采用脉冲场凝胶电泳方法对不同菌株进行分子分型研究,结果供试11株褪色沙雷氏菌的基因相似性在64%~100%之间,相互存在不同程度的亲缘关系。本研究表明,来源于养殖蝗虫的褪色沙雷氏菌野生菌株,具有比较典型的褪色沙雷氏菌生物学性状和系统发育特征,但存在不同的抗原特异性,脉冲场凝胶电泳分析存在一定程度的亲缘关系,对养殖蝗虫具有重要的病原学意义。这些,不仅丰富了褪色沙雷氏菌的生物学性状内容,也为对褪色沙雷氏菌的检验与进一步深入研究提供了具有参考价值的科学资料。另一方面是鉴于褪色沙雷氏菌并非人类及养殖动物的重要病原菌,可能会成为对蝗灾进行生物学防治研究的直接或构建基因工程菌的候选菌株。
[Abstract]:The main biological characters of (Serratia marcescens), isolated from locusts (locusts) isolated from some economic insect farms in Hebei province were studied systematically, including morphological characteristics, physicochemical characteristics, and physical and chemical characteristics. Phylogeny, etiological significance, serological typing, pulse field gel electrophoresis molecular typing and so on. The results showed that the strains from the diseased (dead) cultured locusts had the same basic epibiological characters and phylogenetic characteristics, but there were strains that produced no red pigment. Through the regression infection experiment of healthy locusts, the etiological significance was determined according to the rule of Koch method. The results showed that the isolated strains had strong pathogenic effect and were also the primary pathogenic bacteria of locusts' natural disease (death). The representative strains isolated from different locust farms were selected and the classical serological agglutination test was used to analyze the serological homology. The results showed that there were homology differences among the different strains. However, there were significant serological cross-reactions. The molecular typing of different strains was studied by pulsed field gel electrophoresis (PFGE). The results showed that the genetic similarity of 11 strains of Serratia discolor was between 64% and 100%, and there was different degree of genetic relationship between them. The results showed that the wild strain of Serratia discolor derived from cultured locusts had typical biological and phylogenetic characteristics, but had different antigen specificity. Pulsed-field gel electrophoresis (PFGE) analysis has a certain degree of phylogenetic relationship, which is of great significance for culturing locusts. These results not only enrich the biological characters of Serratia discolor, but also provide valuable scientific data for the examination and further study of Serratia discolor. On the other hand, Serratia discolor is not an important pathogen in human and farmed animals, so it may become a candidate strain to study the biological control of locust infestation directly or to construct genetically engineered bacteria.
【学位授予单位】：河北科技师范学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.5
本文编号：2431475
[Abstract]:The main biological characters of (Serratia marcescens), isolated from locusts (locusts) isolated from some economic insect farms in Hebei province were studied systematically, including morphological characteristics, physicochemical characteristics, and physical and chemical characteristics. Phylogeny, etiological significance, serological typing, pulse field gel electrophoresis molecular typing and so on. The results showed that the strains from the diseased (dead) cultured locusts had the same basic epibiological characters and phylogenetic characteristics, but there were strains that produced no red pigment. Through the regression infection experiment of healthy locusts, the etiological significance was determined according to the rule of Koch method. The results showed that the isolated strains had strong pathogenic effect and were also the primary pathogenic bacteria of locusts' natural disease (death). The representative strains isolated from different locust farms were selected and the classical serological agglutination test was used to analyze the serological homology. The results showed that there were homology differences among the different strains. However, there were significant serological cross-reactions. The molecular typing of different strains was studied by pulsed field gel electrophoresis (PFGE). The results showed that the genetic similarity of 11 strains of Serratia discolor was between 64% and 100%, and there was different degree of genetic relationship between them. The results showed that the wild strain of Serratia discolor derived from cultured locusts had typical biological and phylogenetic characteristics, but had different antigen specificity. Pulsed-field gel electrophoresis (PFGE) analysis has a certain degree of phylogenetic relationship, which is of great significance for culturing locusts. These results not only enrich the biological characters of Serratia discolor, but also provide valuable scientific data for the examination and further study of Serratia discolor. On the other hand, Serratia discolor is not an important pathogen in human and farmed animals, so it may become a candidate strain to study the biological control of locust infestation directly or to construct genetically engineered bacteria.
【学位授予单位】：河北科技师范学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.5
【参考文献】
相关期刊论文 前5条
1 尚艳霞，，张丽娜;粘质沙雷氏菌感染5例临床分析[J];河北医药;1994年02期
2 冯嘉丽;胡静;陈清;;香港海鸥形菌DNA脉冲场凝胶电泳的技术条件[J];热带医学杂志;2010年10期
3 冯广达;邓名荣;郭俊;朱红惠;羊宋贞;梁浩亮;朱昌雄;;广东某农村塘坝饮用水污染的微生物溯源[J];中国环境科学;2011年01期
4 叶蕊;石丽媛;王鹏;宋志忠;;脉冲场凝胶电泳技术简介及其在细菌分子分型中的应用[J];中国媒介生物学及控制杂志;2013年02期
5 吕国平;李丽婕;芦飞;徐保红;剧慧栋;郭玉梅;秦丽云;;食源性金黄色葡萄球菌脉冲场凝胶电泳分型分析[J];微生物学杂志;2013年04期
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