血液核酸筛查非特异扩增曲线的原因分析
发布时间：2019-03-13 11:22
【摘要】：目的分析血液筛查Roche Cobas s201核酸检测系统非特异扩增曲线产生的原因。方法统计本室2014年10月-2015年12月用Cobas Taq Screen MPX v2.0核酸试剂检测产生非特异扩增曲线的次数,探讨其发生与时间、检测系统(A、B和C系统)和试剂批号(批号1、批号2、批号3、批号4、批号5和批号6)之间的关系。结果本室核酸检测非特异扩增曲线总发生率为0.386%(86/2 228);不同月份(χ~2=27.325,P0.05)、不同检测系统(χ~2=66.305,P0.05)和不同批号试剂(χ~2=129.550,P0.05)之间的非特异扩增曲线发生率均有差异,其中2015年2月份、C检测系统和试剂批号2的发生率在各分项中均最大,分别为0.893%(10/1 120)、3.17%(10/315)和5.26%(10/190);非特异扩增曲线类型中以HCV曲线出现47次最多,占54.65%(47/86)。结论核酸检测非特异扩增曲线的发生与检测系统和试剂批号有明显相关性,加强对检测系统的管理和试剂确认管理能够提高核酸检测性能。
[Abstract]:Objective to analyze the causes of nonspecific amplification curve of Roche Cobas S201 nucleic acid detection system in blood screening. Methods from October 2014 to December 2015, the number of non-specific amplification curves produced by Cobas Taq Screen MPX v2.0 nucleic acid reagent was counted, and its occurrence and time were discussed. The detection system (A, B and C systems) and the batch number (lot number 1, B and C) were used to detect the non-specific amplification curve. The relationship between lot number 2, batch number 3, batch number 4, batch number 5 and batch number 6). Results the total incidence of nonspecific amplification curve was 0.386% (86 渭 2.228). The incidence of non-specific amplification curves in different months (蠂 ~ 2 / 27.325, P 0.05), different detection systems (蠂 ~ 2 / 66.305, P 0.05) and different batches of reagents (蠂 ~ 2 / 129.550, P 0.05) were all different, in which February 2015, the incidence of non-specific amplification curve was significantly different. The incidence of C detection system and assay number 2 was the highest in all sub-items, which were 0.893% (10 / 120), 3.17% (10 / 315) and 5.26% (10 / 190), respectively. Among the non-specific amplification curve types, the HCV curve appeared 47 times, accounting for 54.65% (47 / 86). Conclusion the occurrence of non-specific amplification curve of nucleic acid detection has obvious correlation with detection system and reagent batch number. Strengthening the management of detection system and reagent confirmation management can improve the performance of nucleic acid detection.
【作者单位】： 东莞市中心血站;
【基金】：东莞市医疗卫生类科技计划一般项目(2015105101245)
【分类号】：R440
，
本文编号：2439344
[Abstract]:Objective to analyze the causes of nonspecific amplification curve of Roche Cobas S201 nucleic acid detection system in blood screening. Methods from October 2014 to December 2015, the number of non-specific amplification curves produced by Cobas Taq Screen MPX v2.0 nucleic acid reagent was counted, and its occurrence and time were discussed. The detection system (A, B and C systems) and the batch number (lot number 1, B and C) were used to detect the non-specific amplification curve. The relationship between lot number 2, batch number 3, batch number 4, batch number 5 and batch number 6). Results the total incidence of nonspecific amplification curve was 0.386% (86 渭 2.228). The incidence of non-specific amplification curves in different months (蠂 ~ 2 / 27.325, P 0.05), different detection systems (蠂 ~ 2 / 66.305, P 0.05) and different batches of reagents (蠂 ~ 2 / 129.550, P 0.05) were all different, in which February 2015, the incidence of non-specific amplification curve was significantly different. The incidence of C detection system and assay number 2 was the highest in all sub-items, which were 0.893% (10 / 120), 3.17% (10 / 315) and 5.26% (10 / 190), respectively. Among the non-specific amplification curve types, the HCV curve appeared 47 times, accounting for 54.65% (47 / 86). Conclusion the occurrence of non-specific amplification curve of nucleic acid detection has obvious correlation with detection system and reagent batch number. Strengthening the management of detection system and reagent confirmation management can improve the performance of nucleic acid detection.
【作者单位】： 东莞市中心血站;
【基金】：东莞市医疗卫生类科技计划一般项目(2015105101245)
【分类号】：R440
，
本文编号：2439344
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