超早期高压氧治疗对脊髓全横断损伤大鼠股骨骨钙、骨生物力学及骨胶原的影响

发布时间：2019-04-11 11:37

【摘要】：目的探讨超早期高压氧治疗对脊髓全横断损伤大鼠股骨骨钙、骨生物力学及骨胶原的影响。方法 75只Sprague-Dawley大鼠随机分为假手术组(n=15)、模型组(n=20)和高压氧组(n=40),其中高压氧组又分为3小时组(高压氧1组,n=20)和12小时组(高压氧2组,n=20)。假手术组仅行椎板切除术,其他三组均行T10水平脊髓完全横向切断术。高压氧1组和高压氧2组分别于术后第3、12小时开始高压氧治疗,10 d为1个疗程,共3个疗程。治疗后,各组取右股骨测定股骨生物力学性能参数,测定骨钙、骨羟脯氨酸(Hyp)含量;HE染色和Masson染色观察骨小梁和骨胶原形态学变化。结果治疗后,与假手术组比较,模型组股骨结构力学和材料力学参数下降(P0.05),骨干重、骨钙及Hyp含量减少(P0.05);骨小梁及骨胶原数目减少,稀疏、断裂、排列不整。与模型组和高压氧2组比较,高压氧1组股骨结构力学和材料力学参数均增高,骨干重、骨钙及Hyp含量增加(P0.05),骨小梁及骨胶原数目增多,连续性较好,排列较整齐。结论超早期高压氧治疗可明显改善脊髓全横断损伤大鼠股骨骨钙含量,改善骨胶原含量及质量,改善生物力学性能。
[Abstract]:Aim to investigate the effects of hyperbaric oxygen therapy on calcium, bone biomechanics and collagen of femur in rats with spinal cord transection injury. Methods 75 Sprague-Dawley rats were randomly divided into three groups: sham operation group (n = 15), model group (n = 20) and hyperbaric oxygen group (n = 40). The rats in hyperbaric oxygen group were divided into 3 hours group (hyperbaric oxygen group 1, n = 20) and 12 hours group (hyperbaric oxygen group 2, n = 20). In the sham operation group, laminectomy was performed only, and the other three groups underwent complete transverse spinal cord resection at T 10 level. Hyperbaric oxygen group 1 and hyperbaric oxygen group 2 began hyperbaric oxygen therapy at 3 and 12 hours after operation respectively, 10 days as a course of treatment, a total of 3 courses. After treatment, the biomechanical parameters of femur, bone calcium and hydroxyproline (Hyp) content were measured, and the morphological changes of trabecular bone and bone collagen were observed by HE staining and Masson staining. Results after treatment, compared with the sham-operated group, the parameters of femoral structural mechanics and material mechanics in the model group decreased (P0.05), bone mass, bone calcium and Hyp decreased (P0.05). The number of trabeculae and collagen decreased, sparse, fractured, and irregular. Compared with model group and hyperbaric oxygen group, the parameters of femoral structural mechanics and material mechanics, bone mass, bone calcium and Hyp content, bone trabecular and collagen number, continuity and arrangement in hyperbaric oxygen group 1 were higher than those in model group and hyperbaric oxygen group (P0.05). Conclusion Hyperbaric oxygen therapy can significantly improve the calcium content, collagen content and biomechanical properties of femur in rats with spinal cord transection injury.
【作者单位】： 中南大学湘雅二医院;
【基金】：湖南省科技厅基金项目(No.2008SK3108)
【分类号】：R651.2;R459.6
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