核酸检测技术在基层血站血液筛查中的应用
发布时间：2019-05-20 05:13
【摘要】：目的:探讨核酸检测技术在基层血站血液筛查中的应用及存在的问题。方法:对2016年3月—2017年3月本血站采用ELISA进行检测后,对检测结果为阴性的无偿献血血液标本进行HIV、HBV、HCV核酸检测,分析检测结果,以了解核酸的检出率和阳性标本的感染性。结果:对21 214例血液标本进行筛查,HBV-DNA阳性标本的共有70例,阴性标本共有21 144例,阳性率为0.329%,本次血液标本中未发现HCV-RNA、HIV-RNA。结论:核酸检测技术应用于基层血站的血液筛查,能够有效提高对血液传播性疾病的筛查水平,配合相关血清学检测,可显著提高临床用血的安全性。
[Abstract]:Objective: to explore the application and existing problems of nucleic acid detection technology in blood screening of grass-roots blood stations. Methods: after ELISA was used in this blood station from March 2016 to March 2017, HIV,HBV,HCV nucleic acid was detected in unpaid blood samples with negative results, and the results were analyzed. In order to understand the detection rate of nucleic acid and the infection of positive specimens. Results: 21 214 blood samples were screened. There were 70 HBV-DNA positive specimens and 21 144 negative samples, the positive rate was 0.329%. No HCV-RNA,HIV-RNA. was found in this blood sample. Conclusion: the application of nucleic acid detection technology in blood screening in grass-roots blood stations can effectively improve the screening level of blood-borne diseases, and can significantly improve the safety of clinical blood use combined with related serological tests.
【作者单位】： 福建省莆田市中心血站;
【分类号】：R440
[Abstract]:Objective: to explore the application and existing problems of nucleic acid detection technology in blood screening of grass-roots blood stations. Methods: after ELISA was used in this blood station from March 2016 to March 2017, HIV,HBV,HCV nucleic acid was detected in unpaid blood samples with negative results, and the results were analyzed. In order to understand the detection rate of nucleic acid and the infection of positive specimens. Results: 21 214 blood samples were screened. There were 70 HBV-DNA positive specimens and 21 144 negative samples, the positive rate was 0.329%. No HCV-RNA,HIV-RNA. was found in this blood sample. Conclusion: the application of nucleic acid detection technology in blood screening in grass-roots blood stations can effectively improve the screening level of blood-borne diseases, and can significantly improve the safety of clinical blood use combined with related serological tests.
【作者单位】： 福建省莆田市中心血站;
【分类号】：R440
【参考文献】
相关期刊论文 前7条
1 秦煈丽;潘登;戴田;杨树龙;张德卓;吴康乐;;血站核酸实验室质量监控及分析[J];中国卫生检验杂志;2017年05期
2 刘文;李东;;核酸检测技术与血液安全[J];山西医药杂志;2016年12期
3 瞿文金;陈秀琼;邓丽娟;钟旭琴;;分析血站开展血液核酸筛查的必要性及可行性[J];临床医药文献电子杂志;2015年02期
4 邹峥嵘;周国平;郑岚;谢云峥;;上海血站血液核酸检测确认工作初探[J];中国输血杂志;2013年09期
5 曹志刚;李霜云;;核酸检测技术在襄阳地区献血筛查中的应用分析[J];临床输血与检验;2013年02期
6 徐晶;;核酸检测技术在南昌地区无偿献血血液筛查中的应用[J];实验与检验医学;2012年05期
7 余谨;毕昊;陆华新;王先广;赵磊;沈钢;;核酸检测技术在酶联免疫吸附法漏检HIV和HBV血样筛查中的应用[J];国际检验医学杂志;2012年08期
【共引文献】
相关期刊论文 前10条
1 吴洪明;;核酸检测技术在基层血站血液筛查中的应用[J];医学理论与实践;2018年01期
2 庞文静;赵素萍;杜勤;;核酸检测技术应用于血站献血者血液筛查中的价值[J];中国农村卫生;2017年24期
3 汤辉;;酶联免疫吸附试验与核酸检测无偿献血血液标本乙型肝炎病毒的对比[J];医疗装备;2017年24期
4 赵春城;张s，
本文编号：2481381
本文链接：https://www.wllwen.com/huliyixuelunwen/2481381.html
最近更新
教材专著
