UPLC-Q-TOF-MS法测定小鼠大脑中4种单胺类神经递质的含量
发布时间：2019-05-30 09:04
【摘要】：建立了超高效液相色谱-四极杆飞行时间质谱法(UPLC-Q-TOF-MS)对小鼠大脑中的多巴胺(DA)、5-羟色氨(5-HT)、二羟基苯乙酸(DOPAC)和高香草酸(HVA)4种单胺类神经递质的含量进行同时测定。样品以30%乙醇溶液(甲酸调至p H 5.0)匀浆处理后,冷冻离心,过滤。在优化色谱-质谱条件下,采用ESI和APCI复合源,正/负离子扫描方式进行数据采集。4种单胺类神经递质在0.5~1 000μg/L范围内呈良好线性关系,相关系数(r)大于0.999 3,方法的检出限为0.5~5.0μg/L,平均回收率为93.3%~101.8%,相对标准偏差为1.1%~3.3%。该方法具有操作简便、高效快速、回收率良好、灵敏度高、重现性好和准确度高等优点,可用于小鼠脑组织内4种单胺类神经递质的含量测定。
[Abstract]:An ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was established for the treatment of dopamine (DA), 5-hydroxytryptamine (5-HT) in the brain of mice. The contents of four monoamine neurotransmitters, dihydroxyphenylacetic acid (DOPAC) and hyaluronic acid (HVA), were determined at the same time. The sample was homogenized with 30% ethanol solution (formic acid to pH 5.0), frozen and centrifuged and filtered. Under the condition of optimized chromatography-mass spectrometry, the data were collected by positive / negative ion scanning with ESI and APCI composite sources. Four monoamine neurotransmitters showed a good linear relationship in the range of 0.5 鈮，
本文编号：2488714
[Abstract]:An ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was established for the treatment of dopamine (DA), 5-hydroxytryptamine (5-HT) in the brain of mice. The contents of four monoamine neurotransmitters, dihydroxyphenylacetic acid (DOPAC) and hyaluronic acid (HVA), were determined at the same time. The sample was homogenized with 30% ethanol solution (formic acid to pH 5.0), frozen and centrifuged and filtered. Under the condition of optimized chromatography-mass spectrometry, the data were collected by positive / negative ion scanning with ESI and APCI composite sources. Four monoamine neurotransmitters showed a good linear relationship in the range of 0.5 鈮，
本文编号：2488714
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