施氏假单胞菌应用于IPS技术的可行性试验研究

发布时间：2018-06-29 01:12

  本文选题：IPS + 施氏假单胞菌　； 参考：《东南大学学报(自然科学版)》2017年01期

【摘要】：为探究利用施氏假单胞菌代谢产物——氮气加固可液化砂土的可行性,对该菌反硝化作用的条件与砂样中产气效能进行了研究,分析了不同碳源对NO_3~--N与NO-2-N还原率的影响以及温度和初始p H值对其最终饱和度、平均产气速率、初始停滞期的影响,并确定了最优碳源、适用温度和初始p H值区间.试验结果表明,柠檬酸钠为最优碳源,该菌在21 h时对NO_3~--N还原率达到100%,NO-2-N存在先累积后还原的过程,并伴有0.82 mmol/L残留浓度.该菌在4~30℃下均可产气.随温度升高,平均产气速率大幅增加,砂样最终饱和度略有降低.恒温20℃且初始p H值为5~9时,该菌可在砂样中顺利产气.中性及碱性环境下砂样的最终饱和度基本一致,酸性环境下砂样的最终饱和度随初始p H值的减小而降低,细菌在砂样中的平均产气速率随初始p H值的下降而减小.与已有微生物气法相比,将该菌应用于IPS技术中具有平均产气速率快、初始停滞期短、工艺简单等优点.
[Abstract]:In order to explore the feasibility of using nitrogen gas, a metabolite of Pseudomonas sp., to reinforce liquefiable sandy soil, the conditions of denitrification and the efficiency of gas production in sand samples were studied. The effects of different carbon sources on the reduction rate of NO-3-N and NO-2-N and the effects of temperature and initial pH value on the final saturation, average gas production rate and initial stasis period are analyzed. The optimum carbon source, applicable temperature and initial pH value range are determined. The results showed that sodium citrate was the best carbon source, and the reduction rate of no _ 3-N reached 100% at 21 h, accompanied by 0.82 mmol 路L ~ (-1) residual concentration of NO-2-N. The strain can produce gas at 4 鈩，

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