用于蛴螬防治的Bt土壤颗粒剂筛选

发布时间：2018-02-12 15:46

本文关键词： 地下害虫蛴螬 Bt 增殖　出处：《中国生物防治学报》2017年01期 　论文类型：期刊论文

【摘要】：为了研发针对地下害虫蛴螬的Bt新型制剂以提高其防治效果,本研究成功构建了能够表达绿色荧光蛋白的菌株Bt185-gfp,筛选出了对Bt增殖效果优良的花生粕用于防治地下害虫专用剂型的开发。花生粕在1%(质量与体积比)情况下与实验室常用LB培养基相比对Bt增殖无显著差异,说明花生粕是一种营养丰富、适合Bt增殖的材料。花生粕加入Bt菌粉压制成颗粒后,在实验室无菌条件下,颗粒剂中Bt菌体最高增殖了30倍左右;而在土壤环境中,菌体在颗粒剂内增殖了40倍左右。生物测定结果显示,在初始Bt菌量相同情况下,颗粒剂的杀虫活性由未加入花生粕的19.44%提高到加入后的61.11%,杀虫效果增强显著。本研究发现膨化处理不会影响Bt增殖效果,且与灭菌的花生粕获得的Bt活菌数量无显著差异。此外,还评估了利用花生粕增殖其他几种生防菌株的能力,结果发现花生粕能够有效增殖这几种菌株。本研究为防治地下害虫和土传病害生物农药新剂型的开发提供了新思路。
[Abstract]:In order to develop a new BT preparation for underground grub, in order to improve its control effect, In this study, a strain Bt185-gfpwhich can express green fluorescent protein was successfully constructed, and peanut meal, which had good effect on BT proliferation, was selected for the development of special dosage form for underground pest control. There was no significant difference in the proliferation of BT between LB culture medium and lab culture medium. The results showed that peanut meal was a nutritious and suitable material for BT proliferation. When peanut meal was added into BT bacteria powder to form granules, the highest number of BT bacteria in granules was about 30 times under laboratory aseptic conditions, but in soil environment, the highest number of BT bacteria was 30 times higher than that in soil. The bacteria proliferated about 40 times in the granules. The bioassay results showed that the initial BT bacteria amount was the same as that of the original BT bacteria. The insecticidal activity of granules increased from 19.44% without peanut meal to 61.11 after adding peanut meal, and the insecticidal effect was significantly enhanced. There was no significant difference in the number of BT bacteria obtained from sterilized peanut meal. In addition, the ability of using peanut meal to proliferate other biocontrol strains was also evaluated. The results showed that peanut meal could effectively proliferate these strains. This study provided a new idea for the development of a new formulation of biological pesticides for the control of underground pests and soil-borne diseases.
【作者单位】：东北农业大学生命科学学院;中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室;沧州市农林科学院植物保护研究所;
【基金】：国家自然科学基金(31301731,31428020)
【分类号】：S433.83

【相似文献】