新型硅基固载材料的制备及固定化酶性能

发布时间：2017-12-28 17:39

本文关键词：新型硅基固载材料的制备及固定化酶性能　出处：《浙江农林大学学报》2016年02期 　论文类型：期刊论文

【摘要】：利用溶胶-凝胶原理,以正硅酸乙酯(TEOS)为原料,3-缩水甘油醚氧丙基三甲氧基硅烷(KH-560)为偶联剂,在酸性条件下发生缩合反应制备新型基质材料,通过红外光谱(FT-IR),固体核磁共振波谱(CP/MAS13C NMR)和X-射线衍射(XRD)对基质载体进行结构表征。通过交联-包埋固定化法制备新型纤维素固载酶,分析研究了固载酶及游离酶酶解不同微观尺度纤维素基质的酶学性能。结果表明:固载酶进行酶促反应的最优p H 4.0,最优温度为60℃,表明固载酶的热稳定性优于游离酶;测得固载酶的米氏常数与游离酶无显著差异;固定化纤维素酶的重复使用性和储存稳定性较游离酶均有较大改善。有关研究可为制备性能优良的固载酶提供一定借鉴。
[Abstract]:By using the principle of sol-gel process using tetraethoxysilane (TEOS) as raw materials, 3- glycidyl ether trimethoxysilane (KH-560) as coupling agent, preparation of a new matrix condensation reaction system under acidic conditions by infrared spectroscopy (FT-IR), solid state NMR spectrum (CP/MAS13C NMR) and X- X-ray diffraction (XRD) were used to characterize the structures of matrix vector. A new cellulase immobilized enzyme was prepared by cross-linking embedding immobilization. The enzymatic properties of cellulosic matrix at different microscale were analyzed by immobilized enzyme and free enzyme. The results showed that the immobilized enzyme of enzymatic reaction and the optimal P H 4, the optimal temperature is 60 DEG C, indicating that the thermal stability is better than that of free enzyme and immobilized enzyme; there was no significant difference between the Michaelis constant and measured free enzyme immobilized enzyme; immobilized cellulase reusability and storage stability than the free enzyme greatly improved all. The related research can provide some reference for the preparation of the good performance of the immobilized enzyme.
【作者单位】：浙江农林大学理学院;
【基金】：浙江省大学生科技创新活动计划暨新苗人才计划资助项目(2014R412026;201111030107)
【分类号】：TB34;Q814.2
【正文快照】： 酶是一类具有高效催化能力的生物活性物质,生命活动中许多复杂而有规律的物质和能量变化均离不开酶的催化作用。其中,纤维素酶是一类能够将纤维素降解为葡萄糖的多组分酶系的总称,现已确定的主要组分为内切型葡聚糖苷酶、外切型葡萄糖苷酶和纤维二糖酶,它们协同作用,最终水解

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