基于多聚腺嘌呤技术构建新型高效硅基SERS传感器及其检测实体水中汞离子的应用

发布时间：2018-01-07 15:31

本文关键词：基于多聚腺嘌呤技术构建新型高效硅基SERS传感器及其检测实体水中汞离子的应用　出处：《苏州大学》2015年硕士论文　论文类型：学位论文

【摘要】：金/银纳米颗粒已被证明为高效的表面增强拉曼散射(Surface-Enhanced Raman Scattering,SERS)基底,其构建的SERS结构因含有热点(hot spots)区域,可产生极大的拉曼增强效应,有助于增强因子(Enhancement Factor,EF)的提高。此外,通过控制纳米颗粒之间的间距,可以有效地形成耦合热点,进一步增强EF。因此,发展新方法构建金银纳米颗粒自组装结构,已成为近年来的研究热题。探索有效可控的方法,合理设计并构建金银纳米颗粒自组装SERS基底,仍然具有挑战性。本文主要发展了一种基于多聚腺嘌呤的技术构建金银纳米颗粒“核-卫星”自组装结构,并将其用于实际体系的拉曼检测应用中,主要研究内容如下:基于多聚腺嘌呤(Poly Adenine,Poly A)技术,我们可控地构建金银纳米颗粒自组装SERS基底,包括对称的液相金-金纳米颗粒(Au-Au Nanoparticles,Au-AuNPs)以及非对称的固相硅基银-金纳米颗粒(Ag-Au Nanoparticles-decorated Silicon Wafers,Ag-AuNPs@Si)“核-卫星”自组装结构。重要的是,改变Poly A嵌段的长度,可以调节DNA的修饰密度,调控“卫星”金纳米颗粒的数目和间隙,从而得到多种可控的自组装结构,其SERS强度可实现初步调控,并且均具有特异性强、重现性好的特点。其中,制备得到的硅基金银纳米颗粒“核-卫星”自组装结构Ag-AuNPs@Si作为高效的SERS传感器,具有灵敏度高、重现性好的特点,被进一步应用于检测实际体系湖水中汞离子的浓度。其检测极限明显低于电感耦合等离子体原子发射光谱法的测量范围。因此,多聚腺嘌呤DNA技术,可有效、简便地构建高效、可控的SERS基底,改善灵敏度的同时,提高其可靠性、重现性,有助于SERS的基本研究和实际体系的应用,例如食品安全、疾病诊断、环境监控等。
[Abstract]:The gold / silver nanoparticles have been demonstrated for efficient surface enhanced Raman scattering (Surface-Enhanced Raman, Scattering, SERS) base, build its SERS structure (hot spots) due to the presence of hot area, can greatly enhance the Raman effect, contribute to the enhancement factor (Enhancement Factor, EF) increased. In addition, the spacing between the control of nanoparticles, can effectively form a coupled hot spot, further enhance EF. therefore, developing new methods to construct self assembled structure of gold and silver nanoparticles, has become a research hot topic in recent years. To explore the method of controllable, rational design and construction of gold and silver nanoparticles self-assembly SERS substrate remains challenging. In this paper, the development of a poly adenine construction of gold and silver nanometer particles "nuclear - satellite" self assembling based structure, and used to the actual inspection system Raman mapping application, the main research contents As follows: Poly adenine (Poly Adenine, Poly based on A technology, we construct) controlled silver nanoparticles self-assembly SERS substrate, including symmetric liquid gold gold nanoparticles (Au-Au Nanoparticles, Au-AuNPs) and solid phase asymmetric silicon silver - gold nanoparticles (Ag-Au Nanoparticles-decorated Silicon Wafers, Ag-AuNPs@Si) "nuclear and satellite structures. It is important to change the Poly A block length, density modification can adjust the DNA, the number of" satellite "regulation and clearance of gold nanoparticles, resulting in various controllable self-assembly structures, the strength of SERS can realize the preliminary regulation, and has strong specificity and reproducibility the characteristics of good. Among them, silicon fund silver nanoparticles prepared by the" nuclear - satellite "self assemble structure of Ag-AuNPs@Si as a SERS sensor, with high sensitivity, good reproducibility, should be further For the concentration of mercury ion detection system in the actual water. The detection limit was significantly lower than the measurement range by inductively coupled plasma atomic emission spectrometry. Therefore, poly + DNA technology, which can effectively and easily build efficient, controllable SERS substrate, improve the sensitivity at the same time, improve the reliability, reproducibility, the application of basic research help SERS and the actual system, such as food security, disease diagnosis, environmental monitoring and so on.

【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：O657.37;TB383.1

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