仿生硅化固定化胰蛋白酶用于控制生物粘泥的研究

发布时间：2018-01-12 06:12

  本文关键词：仿生硅化固定化胰蛋白酶用于控制生物粘泥的研究　出处：《食品工业科技》2017年09期 　论文类型：期刊论文

  更多相关文章： 胰蛋白酶 仿生硅化 介孔氧化硅 生物粘泥

【摘要】：目的:采用仿生硅化法实现对胰蛋白酶的固定,并将固定化酶与聚甲基丙烯酸甲酯(PMMA)相结合用于抗生物粘泥复合膜的制备。方法:研究了正硅酸甲酯(TMOS)与酶的浓度比、介孔氧化硅(MPS)与酶的质量比、有机溶剂等因素的影响。并以MPS固定化酶为对照实验,对复合膜酶的稳定性、催化活性、重复使用性、抗蛋白质吸附性能等进行了分析。结果:通过仿生硅化法制备的仿生氧化硅-酶-聚甲基丙烯酸甲酯复合膜(Si O_2-Enzyme-PMMA film),与介孔氧化硅-酶-聚甲基丙烯酸甲酯复合膜(MPS-Enzyme-PMMA film)相比,具有更好的稳定性,在25℃和50℃储藏30 d后,两种膜酶活性分别为86.3%和81.2%。Si O_2-Enzyme-PMMA film在重复使用6次之后,复合膜的酶活剩余89.1%。将复合膜浸泡在牛血清白蛋白(BSA)溶液中18 d后,其表面蛋白质吸附量仅为不含酶PMMA的三分之一。两种复合膜均具有良好的抗蛋白吸附性能。结论:仿生硅化制备固定化酶,与介孔氧化硅载体相比,在提高固定化酶的包埋率和稳定性方面有更显著的优势,其条件温和,操作简便,活性高,应用于抗生物粘泥涂层具有更好的防污效果。
[Abstract]:Objective: to immobilize trypsin by bionic silicification. The immobilized enzyme and polymethyl methacrylate (PMMA) were combined to prepare the composite membrane. Methods: the concentration ratio of methyl orthosilicate (TMOS) to polymethyl methacrylate (PMMA) was studied. The effect of mass ratio of mesoporous silica to enzyme, organic solvent and so on. The stability, catalytic activity and reusability of the composite membrane enzyme were studied by using MPS immobilized enzyme as control. Results: the bionic silica-enzyme-poly (methyl methacrylate) composite membranes were prepared by bionic silicification. SiO2-Enzyme-PMMA film. The stability of MPS-Enzyme-PMMA film was better than that of MPS-Enzyme-PMMA film, which was stored at 25 鈩，

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