金纳米颗粒引起高尔基体碎裂导致细胞黏附性降低和肾纤维化

发布时间：2018-01-14 21:00

本文关键词：金纳米颗粒引起高尔基体碎裂导致细胞黏附性降低和肾纤维化　出处：《河北大学》2017年硕士论文　论文类型：学位论文

【摘要】：纳米技术是一类以应用为主的科学,其目的在于研究物质在纳米尺寸(1 nm=10-9 m)下的设计方法、组成、特性以及应用。伴随着纳米技术飞速发展,纳米材料已成功应用于很多领域,包括生物医学、新能源交通、国防建设等等。这就意味着人类暴露在纳米材料环境下的机会在不断增加。研究数据显示,当纳米颗粒通过各种途径进入人体循环后,会在肾脏大量蓄积,使肾脏发生损伤的几率大大增加。其中,金纳米颗粒由于在各个领域的广泛应用,常被作为代表性纳米颗粒来研究其生物学效应。在此,我们制备了从大到小五种不同粒径(10 nm、25 nm、50 nm、70 nm、100 nm)的金纳米颗粒,目的在于探究纳米材料在亚细胞器水平所造成的损伤,和尺寸效应在其中存在的影响。结果发现,金纳米颗粒会通过增大溶酶体膜通透性,引发细胞内的钙库——内质网释放出其中大量的钙离子,从而导致高尔基体应激,使蛋白质糖基化过程出现障碍,表现为肾细胞的黏附性降低及增大肾部纤维化程度。通过本项研究,我们揭示了粒径大于50 nm的金纳米颗粒,会对正常肾细胞中的蛋白质合成相关细胞器造成损伤,最终阻碍了糖蛋白正常表达。不同粒径金纳米颗粒引起溶酶体损伤并导致蛋白质合成相关细胞器应激的研究有助于完善纳米材料与细胞相互作用的明确生物学机制,可以为纳米材料的细胞毒理学、细胞干预研究等提供新思路,也可以对纳米材料的应用和发展提供良好的指导作用。
[Abstract]:Nanotechnology is a kind of application of science, the aim is to study the material in nano size (1 nm=10-9 m) composition design method, the characteristic and application. With the rapid development of nanotechnology, nano materials have been successfully applied in many fields, including biomedicine, new energy and transportation, the national defense construction. Means that human exposure to environmental nano materials under the opportunity to continue to increase. The research data show that when the nano particles into the circulation of the human body through a variety of ways, will accumulate in the kidney, the kidney damage probability increased. Among them, the gold nanoparticles are widely used in various fields, often to study the as a representative of the biological effects of nanoparticles. Then, we prepared from large to small sizes (10 nm, 25 nm, 50 nm, 70 nm, 100 nm) gold nanoparticles, the purpose is to explore the nano materials At the subcellular level caused by the injury, and the size effect in which the influence exists. The results showed that gold nanoparticles by increasing the permeability of the lysosomal membrane, triggering intracellular calcium release from endoplasmic reticulum pool in which a large number of calcium ions, resulting in Golgi body stress, the protein glycosylation process obstacles, for adhesion of renal cells decreased and increased renal fibrosis. Through this study, we reveal the gold nano particle diameter greater than 50 nm, the normal renal cell protein synthesis related organelles damage, ultimately affect the normal expression of glycoprotein. Different sizes of gold nanoparticles by lysosomes study on the damage and lead to protein synthesis related organelles stress is helpful to improve the biological mechanism of nano materials and clear cell interaction, cell toxicity for nano materials science, Cell intervention research provides new ideas, and can also provide a good guidance for the application and development of nanomaterials.

【学位授予单位】：河北大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;R692

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